PMID- 2852578
OWN - NLM
STAT- MEDLINE
DCOM- 19890418
LR  - 20190828
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 7
IP  - 12
DP  - 1988 Dec
TI  - Variation in the number of sites of latency of herpes simplex virus in trigeminal 
      ganglia of inbred mice.
PG  - 1155-62
AB  - Following uniocular topical corneal inoculation with herpes simplex virus type 1 
      (HSV), 176-fold more virus was recovered by 14-day cultivation in vitro from 
      latently infected ipsilateral trigeminal ganglia (TG) of BALB/c mice than from TG 
      of C57BL/6 mice (p = 0.002). Since these quantitative differences may reflect a 
      difference in the number of latently infected cells or a difference in the 
      ability of virus to replicate in secondarily infected cells during cultivation in 
      vitro, experiments were designed to estimate the actual number of sites of 
      latency. Two to four months after topical corneal inoculation, when no active 
      ocular disease was present, minced TG were digested with 2% collagenase. The 
      dissociated cells were placed on monolayers of vero cells, incubated at 31 
      degrees C, and observed for cytopathic effect (CPE) for 14 days. Ipsilateral TG 
      from BALB/c mice produced five-fold more foci of infection than TG from C57BL/6 
      mice (p = 0.007). The number of foci of infection was also dependent upon the 
      dose of virus used to infect the eye. Following infection with high doses of HSV, 
      virus was reactivated from contralateral TG, but in lower numbers than from 
      ipsilateral TG. In vitro studies showed that the replication of virus in ganglia 
      from BALB/c mice was 3-8-fold greater than that in ganglia from C57BL/6 mice. 
      These data support the hypothesis that host genetic factors and the number of 
      infectious particles inoculated influence the number of latently infected cells 
      in the trigeminal ganglion after corneal infection with HSV.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Abghari, S Z
AU  - Abghari SZ
AD  - Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA 
      30322.
FAU - Stulting, R D
AU  - Stulting RD
LA  - eng
GR  - EY05097/EY/NEI NIH HHS/United States
GR  - EY07092/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
SB  - IM
MH  - Animals
MH  - Cornea/microbiology
MH  - Keratitis, Dendritic/microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Simplexvirus/*pathogenicity
MH  - Trigeminal Ganglion/*microbiology
MH  - Trigeminal Nerve/*microbiology
MH  - Virus Activation
MH  - Virus Replication
EDAT- 1988/12/01 00:00
MHDA- 1988/12/01 00:01
CRDT- 1988/12/01 00:00
PHST- 1988/12/01 00:00 [pubmed]
PHST- 1988/12/01 00:01 [medline]
PHST- 1988/12/01 00:00 [entrez]
AID - 10.3109/02713688809033219 [doi]
PST - ppublish
SO  - Curr Eye Res. 1988 Dec;7(12):1155-62. doi: 10.3109/02713688809033219.