PMID- 28525789
OWN - NLM
STAT- MEDLINE
DCOM- 20180205
LR  - 20181202
IS  - 1872-7123 (Electronic)
IS  - 0165-1781 (Linking)
VI  - 254
DP  - 2017 Aug
TI  - Measuring BDNF in saliva using commercial ELISA: Results from a small pilot 
      study.
PG  - 340-346
LID - S0165-1781(16)31094-0 [pii]
LID - 10.1016/j.psychres.2017.04.034 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a protein often studied in 
      psychiatric populations. Commercial ELISA kits have been validated for measuring 
      BDNF in blood plasma and serum, but blood collection is an invasive method which 
      cannot always be used. The aim of this pilot study was to explore the noninvasive 
      alternative of measuring BDNF in saliva. Three different commercial ELISA kits 
      were used to analyze parallel plasma and saliva samples from six healthy adults. 
      In total 33 plasma and 33 saliva samples were analyzed according to 
      manufacturers' standard protocols. BDNF was successfully measured in plasma in 
      two of the three kits, of which the results correlated highly (r(s) =.88). BDNF 
      could not be measured reliably in saliva. The results of this pilot study suggest 
      that techniques of commercial BDNF kits may not be ready for noninvasive saliva 
      measurements, which limits the sampling frequency and settings.
CI  - Copyright (c) 2017 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Vrijen, Charlotte
AU  - Vrijen C
AD  - University of Groningen, University Medical Center Groningen, Interdisciplinary 
      Center Psychopathology and Emotion regulation, CC72, P.O. Box 30001, 9700 RB 
      Groningen, The Netherlands. Electronic address: C.Vrijen@umcg.nl.
FAU - Schenk, Hendrika M
AU  - Schenk HM
AD  - University of Groningen, University Medical Center Groningen, Interdisciplinary 
      Center Psychopathology and Emotion regulation, CC72, P.O. Box 30001, 9700 RB 
      Groningen, The Netherlands. Electronic address: H.M.Schenk@umcg.nl.
FAU - Hartman, Catharina A
AU  - Hartman CA
AD  - University of Groningen, University Medical Center Groningen, Interdisciplinary 
      Center Psychopathology and Emotion regulation, CC72, P.O. Box 30001, 9700 RB 
      Groningen, The Netherlands. Electronic address: C.Hartman@accare.nl.
FAU - Oldehinkel, Albertine J
AU  - Oldehinkel AJ
AD  - University of Groningen, University Medical Center Groningen, Interdisciplinary 
      Center Psychopathology and Emotion regulation, CC72, P.O. Box 30001, 9700 RB 
      Groningen, The Netherlands. Electronic address: A.J.Oldehinkel@umcg.nl.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170422
PL  - Ireland
TA  - Psychiatry Res
JT  - Psychiatry research
JID - 7911385
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/*analysis
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Female
MH  - Healthy Volunteers
MH  - Humans
MH  - Male
MH  - Pilot Projects
MH  - Reproducibility of Results
MH  - Saliva/*chemistry
OTO - NOTNLM
OT  - BDNF
OT  - ELISA
OT  - Plasma
OT  - Saliva
EDAT- 2017/05/20 06:00
MHDA- 2018/02/06 06:00
CRDT- 2017/05/20 06:00
PHST- 2016/06/27 00:00 [received]
PHST- 2017/01/19 00:00 [revised]
PHST- 2017/04/09 00:00 [accepted]
PHST- 2017/05/20 06:00 [pubmed]
PHST- 2018/02/06 06:00 [medline]
PHST- 2017/05/20 06:00 [entrez]
AID - S0165-1781(16)31094-0 [pii]
AID - 10.1016/j.psychres.2017.04.034 [doi]
PST - ppublish
SO  - Psychiatry Res. 2017 Aug;254:340-346. doi: 10.1016/j.psychres.2017.04.034. Epub 
      2017 Apr 22.