PMID- 28526964 OWN - NLM STAT- MEDLINE DCOM- 20171213 LR - 20180710 IS - 1432-8798 (Electronic) IS - 0304-8608 (Linking) VI - 162 IP - 9 DP - 2017 Sep TI - Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China. PG - 2809-2814 LID - 10.1007/s00705-017-3402-5 [doi] AB - A new isolate, 14YV733, of pepper chlorotic spot virus (PCSV) from chili peppers in Yunnan province of China was identified. The typical tospoviral particles of 80-120 nm in diameter were observed by electron microscopy. The virus caused systemic symptoms in several solanaceous plants and the Brassica rapa L. Chinensis group with mechanical inoculation. The sap from infected leaves reacted positively to a rabbit antibody to the N protein of watermelon silver mottle virus (WSMoV) in immunoblotting. The S, M, and L RNAs of PCSV-14YV733 are 3310 nts, 4711 nts, and 8913 nts long, respectively. This is the first report of complete sequences of PCSV in mainland China. Phylogenetic analysis of all tospoviral proteins indicated that PCSV-14YV733 is closely related to members of the WSMoV serogroup. FAU - Zheng, Kuanyu AU - Zheng K AD - Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China. FAU - Chen, Tsung-Chi AU - Chen TC AD - Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan. AD - Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan. FAU - Yeh, Shyi-Dong AU - Yeh SD AD - Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan. FAU - Rahman, Md Siddiqur AU - Rahman MS AD - Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China. AD - Bangladesh Agricultural Research Institute, Gazipur, 1701, Bangladesh. FAU - Su, Xiaoxia AU - Su X AD - Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China. FAU - Wu, Kuo AU - Wu K AD - Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China. FAU - Li, Ting AU - Li T AD - Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China. FAU - Zhang, Zhongkai AU - Zhang Z AD - Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China. zhongkai99@sina.com. FAU - Dong, Jiahong AU - Dong J AD - Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China. dongjhn@126.com. LA - eng GR - 31371919/National Natural Science Foundation of China/ GR - 2013HA028/Top Scientists Input Program of Yunnan/ GR - YBRI201505/Yunnan Provincial Key Lab of Agricultural Biotechnology/ GR - YBRI201501/Yunnan Provincial Key Lab of Agricultural Biotechnology/ PT - Journal Article DEP - 20170519 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (RNA, Viral) SB - IM MH - Brassica rapa/virology MH - Capsicum/*virology MH - China MH - Phylogeny MH - Plant Diseases MH - Plant Viruses/*classification/*genetics MH - RNA, Viral/genetics MH - Solanaceae/virology OTO - NOTNLM OT - Impatiens Necrotic Spot Virus OT - Iris Yellow Spot Virus OT - Melon Yellow Spot Virus OT - Tomato Spot Wilt Virus EDAT- 2017/05/21 06:00 MHDA- 2017/12/14 06:00 CRDT- 2017/05/21 06:00 PHST- 2016/10/22 00:00 [received] PHST- 2017/04/03 00:00 [accepted] PHST- 2017/05/21 06:00 [pubmed] PHST- 2017/12/14 06:00 [medline] PHST- 2017/05/21 06:00 [entrez] AID - 10.1007/s00705-017-3402-5 [pii] AID - 10.1007/s00705-017-3402-5 [doi] PST - ppublish SO - Arch Virol. 2017 Sep;162(9):2809-2814. doi: 10.1007/s00705-017-3402-5. Epub 2017 May 19.