PMID- 28527108 OWN - NLM STAT- MEDLINE DCOM- 20190218 LR - 20220317 IS - 1559-1182 (Electronic) IS - 0893-7648 (Linking) VI - 55 IP - 5 DP - 2018 May TI - Increased Expression of Brain-Derived Neurotrophic Factor Transcripts I and VI, cAMP Response Element Binding, and Glucocorticoid Receptor in the Cortex of Patients with Temporal Lobe Epilepsy. PG - 3698-3708 LID - 10.1007/s12035-017-0597-0 [doi] AB - A body of evidence supports a relevant role of brain-derived neurotrophic factor (BDNF) in temporal lobe epilepsy (TLE). Magnetic resonance data reveal that the cerebral atrophy extends to regions that are functionally and anatomically connected with the hippocampus, especially the temporal cortex. We previously reported an increased expression of BDNF messenger for the exon VI in the hippocampus of temporal lobe epilepsy patients compared to an autopsy control group. Altered levels of this particular transcript were also associated with pre-surgical use of certain psychotropic. We extended here our analysis of transcripts I, II, IV, and VI to the temporal cortex since this cerebral region holds intrinsic communication with the hippocampus and is structurally affected in patients with TLE. We also assayed the cyclic adenosine monophosphate response element-binding (CREB) and glucocorticoid receptor (GR) genes as there is experimental evidence of changes in their expression associated with BDNF and epilepsy. TLE and pre-surgical pharmacological treatment were considered as the primary clinical independent variables. Transcripts BDNF I and BDNF VI increased in the temporal cortex of patients with pharmacoresistant TLE. The expression of CREB and GR expression follow the same direction. Pre-surgical use of selective serotonin reuptake inhibitors, carbamazepine (CBZ) and valproate (VPA), was associated with the differential expression of specific BDNF transcripts and CREB and GR genes. These changes could have functional implication in the plasticity mechanisms related to temporal lobe epilepsy. FAU - Martinez-Levy, G A AU - Martinez-Levy GA AD - Department of Genetics, National Institute of Psychiatry "Ramon de la Fuente Muniz" (INPRFM), Mexico City, Mexico. FAU - Rocha, L AU - Rocha L AD - Department of Pharmacobiology, Center for Research and Advanced Studies, CINVESTAV, Mexico City, Mexico. FAU - Rodriguez-Pineda, F AU - Rodriguez-Pineda F AD - Department of Genetics, National Institute of Psychiatry "Ramon de la Fuente Muniz" (INPRFM), Mexico City, Mexico. FAU - Alonso-Vanegas, M A AU - Alonso-Vanegas MA AD - Neurosurgery Section, National Institute of Neurology and Neurosurgery "Manuel Velasco Suarez" (INNNMVS), Mexico City, Mexico. FAU - Nani, A AU - Nani A AD - Department of Genetics, National Institute of Psychiatry "Ramon de la Fuente Muniz" (INPRFM), Mexico City, Mexico. FAU - Buentello-Garcia, R M AU - Buentello-Garcia RM AD - Neurosurgery Section, National Institute of Neurology and Neurosurgery "Manuel Velasco Suarez" (INNNMVS), Mexico City, Mexico. FAU - Briones-Velasco, M AU - Briones-Velasco M AD - Department of Genetics, National Institute of Psychiatry "Ramon de la Fuente Muniz" (INPRFM), Mexico City, Mexico. FAU - San-Juan, D AU - San-Juan D AD - Clinical Research Department, National Institute of Neurology and Neurosurgery "Manuel Velasco Suarez" (INNNMVS), Mexico City, Mexico. FAU - Cienfuegos, J AU - Cienfuegos J AD - Neurosurgery Section, National Institute of Neurology and Neurosurgery "Manuel Velasco Suarez" (INNNMVS), Mexico City, Mexico. FAU - Cruz-Fuentes, C S AU - Cruz-Fuentes CS AD - Department of Genetics, National Institute of Psychiatry "Ramon de la Fuente Muniz" (INPRFM), Mexico City, Mexico. cruz@imp.edu.mx. LA - eng PT - Journal Article DEP - 20170519 PL - United States TA - Mol Neurobiol JT - Molecular neurobiology JID - 8900963 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cyclic AMP Response Element-Binding Protein) RN - 0 (Receptors, Glucocorticoid) SB - IM MH - Adolescent MH - Aged MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Cerebral Cortex/*metabolism MH - Cyclic AMP Response Element-Binding Protein/genetics/*metabolism MH - Epilepsy, Temporal Lobe/genetics/*metabolism MH - Female MH - Humans MH - Male MH - Middle Aged MH - Receptors, Glucocorticoid/genetics/*metabolism MH - Young Adult OTO - NOTNLM OT - BDNF OT - CREB OT - GR OT - Pharmacoresistant epilepsy OT - Temporal cortex EDAT- 2017/05/21 06:00 MHDA- 2019/02/20 06:00 CRDT- 2017/05/21 06:00 PHST- 2017/02/23 00:00 [received] PHST- 2017/05/03 00:00 [accepted] PHST- 2017/05/21 06:00 [pubmed] PHST- 2019/02/20 06:00 [medline] PHST- 2017/05/21 06:00 [entrez] AID - 10.1007/s12035-017-0597-0 [pii] AID - 10.1007/s12035-017-0597-0 [doi] PST - ppublish SO - Mol Neurobiol. 2018 May;55(5):3698-3708. doi: 10.1007/s12035-017-0597-0. Epub 2017 May 19.