PMID- 28527896
OWN - NLM
STAT- MEDLINE
DCOM- 20171124
LR  - 20171128
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 66
DP  - 2017 Jul
TI  - Molecular characterization and expression analyses of the Solea senegalensis 
      interferon-stimulated gene 15 (isg15) following NNV infections.
PG  - 423-432
LID - S1050-4648(17)30285-1 [pii]
LID - 10.1016/j.fsi.2017.05.040 [doi]
AB  - Interferons are essential in fish resistance to viral infections. They induce 
      interferon-stimulated genes, such as isg15. In this study, the Senegalese sole 
      isg15 gene (ssisg15) has been characterized. As other isg15, ssisg15 contains a 
      402-bp intron sited in the 5'-UTR, and the full length cDNA is 1492-bp, including 
      a 480-bp ORF. The expression analyses revealed basal levels of isg15 transcripts, 
      and a clear induction after poly I:C injection, that reached maximum values in 
      brain, head kidney and gills. The ssisg15 induction patterns were similar in 
      RGNNV- and SJNNV-inoculated fish, whereas the reassortant (RG/SJ) isolate, which 
      has higher replication fitness, triggered delayed but higher transcript levels. 
      Furthermore, RG/SJ infection after poly I:C treatment reduced the induction of 
      ssisg15 transcripts, suggesting an antagonistic mechanism against interferon type 
      I system, that might allow an efficient viral replication at the initial steps of 
      the infective process.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Alvarez-Torres, Daniel
AU  - Alvarez-Torres D
AD  - Universidad de Malaga, Departamento de Microbiologia, Facultad de Ciencias, 
      Campus de Teatinos s/n, 29071 Malaga, Spain; Universidad de Malaga, Departamento 
      de Genetica, Facultad de Ciencias, Campus de Teatinos s/n, 29071 Malaga, Spain.
FAU - Podadera, Ana Maria
AU  - Podadera AM
AD  - Universidad de Malaga, Departamento de Microbiologia, Facultad de Ciencias, 
      Campus de Teatinos s/n, 29071 Malaga, Spain.
FAU - Alonso, M Carmen
AU  - Alonso MC
AD  - Universidad de Malaga, Departamento de Microbiologia, Facultad de Ciencias, 
      Campus de Teatinos s/n, 29071 Malaga, Spain.
FAU - Bandin, Isabel
AU  - Bandin I
AD  - Universidad de Santiago de Compostela, Departamento de Microbiologia, Instituto 
      de Acuicultura, 15782 Santiago de Compostela, Spain.
FAU - Bejar, Julia
AU  - Bejar J
AD  - Universidad de Malaga, Departamento de Genetica, Facultad de Ciencias, Campus de 
      Teatinos s/n, 29071 Malaga, Spain.
FAU - Garcia-Rosado, Esther
AU  - Garcia-Rosado E
AD  - Universidad de Malaga, Departamento de Microbiologia, Facultad de Ciencias, 
      Campus de Teatinos s/n, 29071 Malaga, Spain. Electronic address: 
      megarciar@uma.es.
LA  - eng
PT  - Journal Article
DEP - 20170517
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ubiquitins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Cytokines/chemistry/*genetics/metabolism
MH  - DNA, Complementary/genetics/metabolism
MH  - Fish Diseases/*genetics/immunology
MH  - Fish Proteins/chemistry/*genetics/metabolism
MH  - *Flatfishes
MH  - Nodaviridae/genetics/*physiology
MH  - Phylogeny
MH  - RNA Virus Infections/genetics/immunology/*veterinary
MH  - RNA, Messenger/genetics/metabolism
MH  - Ubiquitins/chemistry/*genetics/metabolism
OTO - NOTNLM
OT  - IFN I
OT  - NNV
OT  - Solea senegalensis
OT  - isg15 gene
EDAT- 2017/05/22 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/05/22 06:00
PHST- 2017/03/29 00:00 [received]
PHST- 2017/05/11 00:00 [revised]
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/05/22 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/05/22 06:00 [entrez]
AID - S1050-4648(17)30285-1 [pii]
AID - 10.1016/j.fsi.2017.05.040 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2017 Jul;66:423-432. doi: 10.1016/j.fsi.2017.05.040. Epub 
      2017 May 17.