PMID- 28528122
OWN - NLM
STAT- MEDLINE
DCOM- 20180328
LR  - 20220311
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1668
DP  - 2017 Aug 1
TI  - ProBDNF inhibits proliferation, migration and differentiation of mouse neural 
      stem cells.
PG  - 46-55
LID - S0006-8993(17)30209-3 [pii]
LID - 10.1016/j.brainres.2017.05.013 [doi]
AB  - ProBDNF, a precursor of brain-derived neurotrophic factor (BDNF), is an important 
      regulator of neurodegeneration, hippocampal long-term depression, and synaptic 
      plasticity. ProBDNF and its receptors pan-neurotrophin receptor p75 (p75NTR), 
      vps10p domain-containing receptor Sortilin and tropomyosin receptor kinase B 
      (TrkB) are expressed in neuronal and glial cells. The role of proBDNF in 
      regulation of neurogenesis is not fully defined. This study aims to uncover the 
      function of proBDNF in regulating the differentiation, migration and 
      proliferation of mouse neural stem cells (NSCs) in vitro. We have found that 
      proBDNF and its receptors are constitutively expressed in NSCs when assessed by 
      immunocytochemistry and western blotting. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 
      diphenyl tetrazolium bromide) assay showed that exogenous proBDNF treatment 
      reduced mouse NSCs viability by 38% at 10ng/mL. The migration of NSCs was also 
      reduced by exogenous proBDNF treatment in a concentration-dependent manner (by 
      90% at 10ng/mL) but increased by anti-proBDNF antibody treatment (by 50%). BrdU 
      (5-Bromo-2'-Deoxyuridine) incorporation was performed for detection of newborn 
      cells. We have found that proBDNF significantly inhibited proliferation of NSCs 
      and reduced the number of differentiated neurons, oligodendrocytes and 
      astrocytes, while anti-proBDNF antibody treatment promoted proliferation and 
      differentiation of NSCs. In conclusion, proBDNF may oppose the functions of 
      mature BDNF by inhibiting the proliferation, differentiation and migration of 
      NSCs during development. Conversely, anti-proBDNF antibody treatment promoted 
      proliferation and differentiation of NSCs.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Li, Jia-Yi
AU  - Li JY
AD  - School of Pharmacy and Medical Sciences, Division of Health Sciences, Sansom 
      Institute for Health Research, University of South Australia, Adelaide 5000, SA, 
      Australia. Electronic address: jiayi.li@mymail.unisa.edu.au.
FAU - Liu, Jia
AU  - Liu J
AD  - Animal Research Centre, Kunming Medical University, Kunming, China.
FAU - Manaph, Nimshitha Pavathuparambil Abdul
AU  - Manaph NPA
AD  - School of Pharmacy and Medical Sciences, Division of Health Sciences, Sansom 
      Institute for Health Research, University of South Australia, Adelaide 5000, SA, 
      Australia.
FAU - Bobrovskaya, Larisa
AU  - Bobrovskaya L
AD  - School of Pharmacy and Medical Sciences, Division of Health Sciences, Sansom 
      Institute for Health Research, University of South Australia, Adelaide 5000, SA, 
      Australia. Electronic address: Larisa.Bobrovskaya@unisa.edu.au.
FAU - Zhou, Xin-Fu
AU  - Zhou XF
AD  - School of Pharmacy and Medical Sciences, Division of Health Sciences, Sansom 
      Institute for Health Research, University of South Australia, Adelaide 5000, SA, 
      Australia.
LA  - eng
PT  - Journal Article
DEP - 20170517
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Precursors)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - Z020Y8WIJ4 (sortilin)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport/pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - *Cell Differentiation/drug effects
MH  - *Cell Movement/drug effects
MH  - *Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Hippocampus/drug effects/metabolism
MH  - Mice, Inbred C57BL
MH  - Neural Stem Cells/*cytology/drug effects
MH  - Oligodendroglia/cytology
MH  - Protein Precursors/metabolism
MH  - Receptor, Nerve Growth Factor/metabolism
OTO - NOTNLM
OT  - Differentiation
OT  - Migration
OT  - Neural stem cell
OT  - ProBDNF
OT  - Proliferation
EDAT- 2017/05/22 06:00
MHDA- 2018/03/29 06:00
CRDT- 2017/05/22 06:00
PHST- 2016/12/18 00:00 [received]
PHST- 2017/04/19 00:00 [revised]
PHST- 2017/05/12 00:00 [accepted]
PHST- 2017/05/22 06:00 [pubmed]
PHST- 2018/03/29 06:00 [medline]
PHST- 2017/05/22 06:00 [entrez]
AID - S0006-8993(17)30209-3 [pii]
AID - 10.1016/j.brainres.2017.05.013 [doi]
PST - ppublish
SO  - Brain Res. 2017 Aug 1;1668:46-55. doi: 10.1016/j.brainres.2017.05.013. Epub 2017 
      May 17.