PMID- 28529565
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 13
IP  - 5
DP  - 2017 May
TI  - Prodelphinidins isolated from Chinese bayberry leaves induces apoptosis via the 
      p53-dependent signaling pathways in OVCAR-3 human ovarian cancer cells.
PG  - 3210-3218
LID - 10.3892/ol.2017.5813 [doi]
AB  - Chinese bayberry leaves are rich in prodelphinidins. Since the isolation and 
      purification of prodelphinidins is difficult, the association between the degree 
      of prodelphinidin polymerization and their anti-carcinogenic activity remains 
      ambiguous. The cytotoxic and apoptotic activities of prodelphinidin Chinese 
      bayberry leaf extracts (PCBLs), oligomeric proanthocyanidins (OPAs) and polymeric 
      proanthocyanidins (PPAs), isolated by normal-phase preparative high-performance 
      liquid chromatography were investigated in OVCAR-3 human ovarian cancer cells. 
      The PCBLs, OPAs and PPAs inhibited cancer cell growth and induced apoptosis via 
      the caspase-dependent pathway. Apoptosis was triggered through the intrinsic 
      pathway by upregulating the expression of several B-cell lymphoma-2 (Bcl-2) 
      family proapoptotic proteins, including p53-upregulated modulator of apoptosis 
      (PUMA), Bcl-2-associated X protein and Bcl-2-associated agonist of cell death, 
      and by downregulating the antiapoptotic protein Bcl-extra large. Apoptosis was 
      also triggered through the extrinsic pathway via the upregulation of death 
      receptor 5 (DR5) and Fas expression. In addition, OPAs and PPAs induced 
      caspase-dependent apoptosis at least partially through the inhibition of the 
      protein kinase B signaling pathway. The knockdown of p53 by specific small 
      interfering RNA resulted in the depletion of p53, and inhibited the OPA and PPA 
      treatment-induced increases in p53, which led to a decrease in the expression of 
      p21, DR5, Fas, PUMA and phosphatase and tensin homolog proteins. These 
      observations demonstrate that p53 is a mediator of OPA and PPA-induced apoptosis 
      in OVCAR-3 cells. The PPAs exhibited stronger anti-proliferative and 
      pro-apoptotic activities compared with OPAs and PCBLs. These results suggest that 
      PCBLs, OPAs and PPAs may be useful for the treatment of ovarian cancer.
FAU - Fu, Yu
AU  - Fu Y
AD  - College of Biosystems Engineering and Food Science, Fuli Institute of Food 
      Science, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China.
AD  - College of Science, Technology and Mathematics, Alderson Broaddus University, 
      Philippi, WV 26416, USA.
FAU - Ye, Xingqian
AU  - Ye X
AD  - College of Biosystems Engineering and Food Science, Fuli Institute of Food 
      Science, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China.
FAU - Lee, Malcolm
AU  - Lee M
AD  - College of Science, Technology and Mathematics, Alderson Broaddus University, 
      Philippi, WV 26416, USA.
FAU - Rankin, Gary
AU  - Rankin G
AD  - Department of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of 
      Medicine, Marshall University, Huntington, WV 25755, USA.
FAU - Chen, Yi Charlie
AU  - Chen YC
AD  - College of Science, Technology and Mathematics, Alderson Broaddus University, 
      Philippi, WV 26416, USA.
LA  - eng
PT  - Journal Article
DEP - 20170306
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5431737
OTO - NOTNLM
OT  - Myrica rubra
OT  - apoptosis
OT  - leaves
OT  - ovarian cancer
OT  - prodelphinidins
EDAT- 2017/05/23 06:00
MHDA- 2017/05/23 06:01
PMCR- 2017/03/06
CRDT- 2017/05/23 06:00
PHST- 2016/04/27 00:00 [received]
PHST- 2016/12/02 00:00 [accepted]
PHST- 2017/05/23 06:00 [entrez]
PHST- 2017/05/23 06:00 [pubmed]
PHST- 2017/05/23 06:01 [medline]
PHST- 2017/03/06 00:00 [pmc-release]
AID - OL-0-0-5813 [pii]
AID - 10.3892/ol.2017.5813 [doi]
PST - ppublish
SO  - Oncol Lett. 2017 May;13(5):3210-3218. doi: 10.3892/ol.2017.5813. Epub 2017 Mar 6.