PMID- 28530470
OWN - NLM
STAT- MEDLINE
DCOM- 20170808
LR  - 20181113
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Print)
IS  - 0022-0345 (Linking)
VI  - 96
IP  - 6
DP  - 2017 Jun
TI  - CD73 Controls Extracellular Adenosine Generation in the Trigeminal Nociceptive 
      Nerves.
PG  - 671-677
LID - 10.1177/0022034517692953 [doi]
AB  - Purinergic signaling is involved in pain generation and modulation in the 
      nociceptive sensory nervous system. Adenosine triphosphate (ATP) induces pain via 
      activation of ionotropic P2X receptors while adenosine mediates analgesia via 
      activation of metabotropic P1 receptors. These purinergic signaling are 
      determined by ecto-nucleotidases that control ATP degradation and adenosine 
      generation. Using enzymatic histochemistry, we detected ecto-AMPase activity in 
      dental pulp, trigeminal ganglia (TG) neurons, and their nerve fibers. Using 
      immunofluorescence staining, we confirmed the expression of ecto-5'-nucleotidase 
      (CD73) in trigeminal nociceptive neurons and their axonal fibers, including the 
      nociceptive nerve fibers projecting into the brainstem. In addition, we detected 
      the existence of CD73 and ecto-AMPase activity in the nociceptive lamina of the 
      trigeminal subnucleus caudalis (TSNC) in the brainstem. Furthermore, we 
      demonstrated that incubation with specific anti-CD73 serum significantly reduced 
      the ecto-AMPase activity in the nociceptive lamina in the brainstem. Our results 
      indicate that CD73 might participate in nociceptive modulation by affecting 
      extracellular adenosine generation in the trigeminal nociceptive pathway. 
      Disruption of TG neuronal ecto-nucleotidase expression and axonal terminal 
      localization under certain circumstances such as chronic inflammation, oxidant 
      stress, local constriction, and injury in trigeminal nerves may contribute to the 
      pathogenesis of orofacial neuropathic pain.
FAU - Liu, X
AU  - Liu X
AD  - 1 Department of Dentistry, Eastman Institute for Oral Health, University of 
      Rochester School of Medicine and Dentistry, Rochester, NY, USA.
AD  - 2 Department of Pharmacology and Physiology, University of Rochester School of 
      Medicine and Dentistry, Rochester, NY, USA.
FAU - Ma, L
AU  - Ma L
AD  - 1 Department of Dentistry, Eastman Institute for Oral Health, University of 
      Rochester School of Medicine and Dentistry, Rochester, NY, USA.
AD  - 3 Department of Dentistry, School of Stomatology, Zhengzhou University, 
      Zhengzhou, China.
FAU - Zhang, S
AU  - Zhang S
AD  - 1 Department of Dentistry, Eastman Institute for Oral Health, University of 
      Rochester School of Medicine and Dentistry, Rochester, NY, USA.
FAU - Ren, Y
AU  - Ren Y
AD  - 1 Department of Dentistry, Eastman Institute for Oral Health, University of 
      Rochester School of Medicine and Dentistry, Rochester, NY, USA.
FAU - Dirksen, R T
AU  - Dirksen RT
AD  - 2 Department of Pharmacology and Physiology, University of Rochester School of 
      Medicine and Dentistry, Rochester, NY, USA.
LA  - eng
GR  - KL2 TR000095/TR/NCATS NIH HHS/United States
GR  - R01 AR059646/AR/NIAMS NIH HHS/United States
PT  - Journal Article
DEP - 20170216
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - EC 3.1.3.5 (5'-Nucleotidase)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - 5'-Nucleotidase/*metabolism/*physiology
MH  - Adenosine Triphosphatases/*metabolism
MH  - Adolescent
MH  - Animals
MH  - Axons/*enzymology
MH  - Brain Stem/*enzymology
MH  - Dental Pulp/cytology/*enzymology
MH  - Facial Pain/enzymology
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Hydrolysis
MH  - Molar, Third
MH  - Nerve Fibers/*enzymology
MH  - Signal Transduction
MH  - Staining and Labeling
MH  - Trigeminal Caudal Nucleus/*enzymology
MH  - Trigeminal Nerve/*enzymology
MH  - Young Adult
PMC - PMC5444617
OTO - NOTNLM
OT  - antinociception
OT  - ecto-nucleotidases
OT  - neuropathic pain
OT  - orofacial pain
OT  - purinergic signaling
OT  - trigeminal nerves
COIS- The authors declare no potential conflicts of interest with respect to the 
      authorship and/or publication of this article.
EDAT- 2017/05/23 06:00
MHDA- 2017/08/09 06:00
PMCR- 2018/06/01
CRDT- 2017/05/23 06:00
PHST- 2017/05/23 06:00 [entrez]
PHST- 2017/05/23 06:00 [pubmed]
PHST- 2017/08/09 06:00 [medline]
PHST- 2018/06/01 00:00 [pmc-release]
AID - 10.1177_0022034517692953 [pii]
AID - 10.1177/0022034517692953 [doi]
PST - ppublish
SO  - J Dent Res. 2017 Jun;96(6):671-677. doi: 10.1177/0022034517692953. Epub 2017 Feb 
      16.