PMID- 28531280
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20181113
IS  - 1758-535X (Electronic)
IS  - 1079-5006 (Print)
IS  - 1079-5006 (Linking)
VI  - 72
IP  - 12
DP  - 2017 Nov 9
TI  - Effects of Calorie Restriction and Fiber Type on Glucose Uptake and Abundance of 
      Electron Transport Chain and Oxidative Phosphorylation Proteins in Single Fibers 
      from Old Rats.
PG  - 1638-1646
LID - 10.1093/gerona/glx099 [doi]
AB  - Calorie restriction (CR; reducing calorie intake by ~40% below ad libitum) can 
      increase glucose uptake by insulin-stimulated muscle. Because skeletal muscle is 
      comprised of multiple, heterogeneous fiber types, our primary aim was to 
      determine the effects of CR (initiated at 14 weeks old) and fiber type on 
      insulin-stimulated glucose uptake by single fibers of diverse fiber types in 
      23-26-month-old rats. Isolated epitrochlearis muscles from AL and CR rats were 
      incubated with [3H]-2-deoxyglucose +/- insulin. Glucose uptake and fiber type were 
      determined for single fibers dissected from the muscles. We also determined 
      CR-effects on abundance of several key metabolic proteins in single fibers. CR 
      resulted in: (a) significantly (p < .05 to .001) greater glucose uptake by 
      insulin-stimulated type I, IIA, IIB, IIBX, and IIX fibers; (b) significantly (p < 
      .05 to .001) reduced abundance of several mitochondrial electron transport chain 
      (ETC) and oxidative phosphorylation (OxPhos) proteins in type I, IIA, and IIBX 
      but not IIB and IIX fibers; and (c) unaltered hexokinase II abundance in each 
      fiber type. These results demonstrate that CR can enhance glucose uptake in each 
      fiber type of rat skeletal muscle in the absence of upregulation of the abundance 
      of hexokinase II or key mitochondrial ETC and OxPhos proteins.
CI  - (c) The Author 2017. Published by Oxford University Press on behalf of The 
      Gerontological Society of America. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Wang, Haiyan
AU  - Wang H
AD  - Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann 
      Arbor.
FAU - Arias, Edward B
AU  - Arias EB
AD  - Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann 
      Arbor.
FAU - Yu, Carmen S
AU  - Yu CS
AD  - Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann 
      Arbor.
FAU - Verkerke, Anthony R P
AU  - Verkerke ARP
AD  - Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann 
      Arbor.
FAU - Cartee, Gregory D
AU  - Cartee GD
AD  - Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann 
      Arbor.
AD  - Department of Molecular and Integrative Physiology, University of Michigan, Ann 
      Arbor.
AD  - Institute of Gerontology, University of Michigan, Ann Arbor.
LA  - eng
GR  - P30 DK020572/DK/NIDDK NIH HHS/United States
GR  - R01 AG010026/AG/NIA NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Gerontol A Biol Sci Med Sci
JT  - The journals of gerontology. Series A, Biological sciences and medical sciences
JID - 9502837
RN  - 0 (Proteins)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - *Caloric Restriction
MH  - Electron Transport/*physiology
MH  - Glucose/*metabolism
MH  - Male
MH  - Muscle Fibers, Skeletal/*metabolism
MH  - *Oxidative Phosphorylation
MH  - Proteins/physiology
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred F344
PMC - PMC5861973
OTO - NOTNLM
OT  - Glucose transport
OT  - Hexokinase II
OT  - Insulin resistance
OT  - Insulin sensitivity
OT  - Mitochondria
EDAT- 2017/05/23 06:00
MHDA- 2017/12/12 06:00
PMCR- 2018/11/09
CRDT- 2017/05/23 06:00
PHST- 2016/12/16 00:00 [received]
PHST- 2017/05/23 06:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
PHST- 2017/05/23 06:00 [entrez]
PHST- 2018/11/09 00:00 [pmc-release]
AID - 3837091 [pii]
AID - glx099 [pii]
AID - 10.1093/gerona/glx099 [doi]
PST - ppublish
SO  - J Gerontol A Biol Sci Med Sci. 2017 Nov 9;72(12):1638-1646. doi: 
      10.1093/gerona/glx099.