PMID- 28532241
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20170726
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 81
IP  - 8
DP  - 2017 Aug
TI  - Bacillus cereus-type polyhydroxyalkanoate biosynthetic gene cluster contains 
      R-specific enoyl-CoA hydratase gene.
PG  - 1627-1635
LID - 10.1080/09168451.2017.1325314 [doi]
AB  - Bacillus cereus and Bacillus megaterium both accumulate polyhydroxyalkanoate 
      (PHA) but their PHA biosynthetic gene (pha) clusters that code for proteins 
      involved in PHA biosynthesis are different. Namely, a gene encoding MaoC-like 
      protein exists in the B. cereus-type pha cluster but not in the B. 
      megaterium-type pha cluster. MaoC-like protein has an R-specific enoyl-CoA 
      hydratase (R-hydratase) activity and is referred to as PhaJ when involved in PHA 
      metabolism. In this study, the pha cluster of B. cereus YB-4 was characterized in 
      terms of PhaJ's function. In an in vitro assay, PhaJ from B. cereus YB-4 
      (PhaJ(YB4)) exhibited hydration activity toward crotonyl-CoA. In an in vivo assay 
      using Escherichia coli as a host for PHA accumulation, the recombinant strain 
      expressing PhaJ(YB4) and PHA synthase led to increased PHA accumulation, 
      suggesting that PhaJ(YB4) functioned as a monomer supplier. The monomer 
      composition of the accumulated PHA reflected the substrate specificity of 
      PhaJ(YB4), which appeared to prefer short chain-length substrates. The pha 
      cluster from B. cereus YB-4 functioned to accumulate PHA in E. coli; however, it 
      did not function when the phaJ(YB4) gene was deleted. The B. cereus-type pha 
      cluster represents a new example of a pha cluster that contains the gene encoding 
      PhaJ.
FAU - Kihara, Takahiro
AU  - Kihara T
AD  - a Department of Materials Science and Engineering, Tokyo Institute of Technology 
      , Midori-ku, Yokohama , Japan.
AD  - b Department of Innovative and Engineered Materials, Tokyo Institute of 
      Technology , Midori-ku, Yokohama , Japan.
FAU - Hiroe, Ayaka
AU  - Hiroe A
AD  - a Department of Materials Science and Engineering, Tokyo Institute of Technology 
      , Midori-ku, Yokohama , Japan.
AD  - b Department of Innovative and Engineered Materials, Tokyo Institute of 
      Technology , Midori-ku, Yokohama , Japan.
FAU - Ishii-Hyakutake, Manami
AU  - Ishii-Hyakutake M
AD  - c Bioplastic Research Team, RIKEN Biomass Engineering Program , Wako-shi, Saitama 
      , Japan.
FAU - Mizuno, Kouhei
AU  - Mizuno K
AD  - d Department of Creative Engineering , National Institute of Technology, 
      Kitakyushu College , Kitakyushu , Japan.
FAU - Tsuge, Takeharu
AU  - Tsuge T
AD  - a Department of Materials Science and Engineering, Tokyo Institute of Technology 
      , Midori-ku, Yokohama , Japan.
LA  - eng
PT  - Journal Article
DEP - 20170522
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Acyl Coenzyme A)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Caproates)
RN  - 0 (Pentanoic Acids)
RN  - 0 (Polyhydroxyalkanoates)
RN  - 0 (Recombinant Proteins)
RN  - 107-92-6 (Butyric Acid)
RN  - 1F8SN134MX (hexanoic acid)
RN  - 992-67-6 (crotonyl-coenzyme A)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase)
RN  - EC 4.2.1.17 (Enoyl-CoA Hydratase)
RN  - GZK92PJM7B (n-pentanoic acid)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acyl Coenzyme A/metabolism
MH  - Acyltransferases/genetics/metabolism
MH  - Bacillus cereus/enzymology/genetics
MH  - Bacillus megaterium/enzymology/genetics
MH  - Bacterial Proteins/genetics/metabolism
MH  - Butyric Acid/metabolism
MH  - Caproates/metabolism
MH  - Cloning, Molecular
MH  - Enoyl-CoA Hydratase/genetics/metabolism
MH  - Escherichia coli/genetics/metabolism
MH  - Gene Expression
MH  - Glucose/metabolism
MH  - *Multigene Family
MH  - Pentanoic Acids/metabolism
MH  - Plasmids/chemistry/metabolism
MH  - Polyhydroxyalkanoates/biosynthesis
MH  - Recombinant Proteins
MH  - Species Specificity
MH  - Substrate Specificity
OTO - NOTNLM
OT  - PhaJ
OT  - R-specific hydratase
OT  - monomer supplier
OT  - polyhydroxyalkanoate
EDAT- 2017/05/24 06:00
MHDA- 2017/07/27 06:00
CRDT- 2017/05/24 06:00
PHST- 2017/05/24 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2017/05/24 06:00 [entrez]
AID - 10.1080/09168451.2017.1325314 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2017 Aug;81(8):1627-1635. doi: 
      10.1080/09168451.2017.1325314. Epub 2017 May 22.