PMID- 28532802
OWN - NLM
STAT- MEDLINE
DCOM- 20180103
LR  - 20220408
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 204
DP  - 2017 May
TI  - Oral fluid versus blood sampling in group-housed sows and finishing pigs: 
      Feasibility and performance of antibody detection for porcine reproductive and 
      respiratory syndrome virus (PRRSV).
PG  - 25-34
LID - S0378-1135(17)30221-3 [pii]
LID - 10.1016/j.vetmic.2017.04.001 [doi]
AB  - The feasibility of using individual and pen-based oral fluid samples to detect 
      PRRSV antibodies in growing-finishing pigs and group-housed sows was 
      investigated. The diagnostic performances of a commercial oral fluid ELISA 
      (OF-ELISA) and a serum ELISA (SER-ELISA) performed on individual or pooled 
      samples from 5 or 10 pigs and sows was evaluated. The performance of the OF-ELISA 
      was also assessed for pen-based oral fluids. Eight hundred and thirty-four pigs 
      and 1598 sows from 42 PRRSV-infected and 3 PRRSV-negative herds were oral fluid 
      sampled and bled. PRRSV antibodies were detected by an OF-ELISA performed at 
      individual, pool (5 or 10 samples) and pen levels. Serum samples were tested by a 
      SER-ELISA at individual and pool levels. The sensitivity and specificity of 
      ELISAs for individual samples were assessed by Bayesian analysis. The relative 
      diagnostic performance for the pools was calculated by taking individual samples 
      as the gold standard. SER-ELISA and individual OF-ELISA results were used as 
      references for estimating OF-ELISA performance for pen-based samples. Individual 
      oral fluid collection was feasible in all kinds of pigs, whereas pen-based 
      samples were unsuccessful in 40% of the group-housed sow pens. High levels of 
      sensitivity comparable to those of the SER-ELISA were found for the OF-ELISA when 
      performed on individual, 5-sample pool or pen-based samples from pigs or sows. 
      The OF-ELISA lacked specificity for individual samples from sows. Pooling 5 
      individual oral fluid samples or using pen-based samples increased test 
      specificity.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Fablet, C
AU  - Fablet C
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France. 
      Electronic address: christelle.fablet@anses.fr.
FAU - Renson, P
AU  - Renson P
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Virologie Immunologie 
      Porcines, B.P. 53, 22440 Ploufragan, France; Union des Groupements de Producteurs 
      de Viande de Bretagne (UGPVB), 104 rue Eugene Pottier, CS 26553, 35065 Rennes, 
      France; Universite Bretagne-Loire, France.
FAU - Pol, F
AU  - Pol F
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Dorenlor, V
AU  - Dorenlor V
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Mahe, S
AU  - Mahe S
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Virologie Immunologie 
      Porcines, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Eono, F
AU  - Eono F
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Eveno, E
AU  - Eveno E
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Le Dimna, M
AU  - Le Dimna M
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Virologie Immunologie 
      Porcines, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Liegard-Vanhecke, D
AU  - Liegard-Vanhecke D
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Eudier, S
AU  - Eudier S
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Rose, N
AU  - Rose N
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Epidemiologie et Bien-Etre 
      du Porc, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
FAU - Bourry, O
AU  - Bourry O
AD  - Agence Nationale de Securite Sanitaire (ANSES), Unite Virologie Immunologie 
      Porcines, B.P. 53, 22440 Ploufragan, France; Universite Bretagne-Loire, France.
LA  - eng
PT  - Journal Article
DEP - 20170404
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood/*chemistry
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Specimen Handling/instrumentation/veterinary
MH  - Swine
OTO - NOTNLM
OT  - Diagnostic performance
OT  - ELISA
OT  - Oral fluid
OT  - PRRSV antibody
OT  - Serum
EDAT- 2017/05/24 06:00
MHDA- 2018/01/04 06:00
CRDT- 2017/05/24 06:00
PHST- 2017/02/17 00:00 [received]
PHST- 2017/04/03 00:00 [accepted]
PHST- 2017/05/24 06:00 [entrez]
PHST- 2017/05/24 06:00 [pubmed]
PHST- 2018/01/04 06:00 [medline]
AID - S0378-1135(17)30221-3 [pii]
AID - 10.1016/j.vetmic.2017.04.001 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 May;204:25-34. doi: 10.1016/j.vetmic.2017.04.001. Epub 2017 
      Apr 4.