PMID- 28533813
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1749-8546 (Print)
IS  - 1749-8546 (Electronic)
IS  - 1749-8546 (Linking)
VI  - 12
DP  - 2017
TI  - Rapid identification of growth years and profiling of bioactive ingredients in 
      Astragalus membranaceus var. mongholicus (Huangqi) roots from Hunyuan, Shanxi.
PG  - 14
LID - 10.1186/s13020-017-0135-z [doi]
LID - 14
AB  - BACKGROUND: The content of medicinal bioactive constituents in huangqi is 
      affected by plant age. In this study, we devised a quick and convenient method 
      for determining the age of huangqi, which was cultivated in Hunyuan County 
      (Shanxi Province). METHODS: 1, 2, 3, 4, 5, 8, 10 growth years huangqi had 38 
      samples, all samples were collected separately. The growth rings in these samples 
      were observed after making paraffin section and freehand-section. The 
      relationship between growth rings and its growth years was analyzed by SPSS 19.0 
      software. Histochemical localization of total flavones and saponins in huangqi 
      was determined by color reactions. The concentration of four flavonoids and two 
      saponins in the roots of huangqi of different ages and different organizational 
      structure (normal roots and rotten heart roots) were determined by HPLC-DAD and 
      HPLC-ELSD. The results were analyzed by SPSS 19.0 software. RESULTS: All huangqi 
      samples had clear growth rings, and the statistical result about growth rings (X) 
      and growth years (Y) showed significant correlation (r = 1, P = 0.000). The 
      calibration curves of these six ingredients showed good linearity respectively, 
      with significant correlation. All relative standard deviations (RSDs) of 
      precision, recovery, repeatability, and stability experiments were less than 2%. 
      Roots of 5-year-old plants contained the highest concentrations of total 
      flavonoids and saponins. Saponin concentrations increased toward the center of 
      the roots, whereas the four flavonoids showed an opposite trend in tissue 
      distribution. CONCLUSION: The growth year of huangqi (Hunyuan County, Shanxi 
      Province) could be determined soon and conveniently by naked eyes after staining 
      phloroglucinol-HCl solution on freehand section. The content of saponins and 
      flavonoids in rotten heart root and the surrounding normal tissues were affected 
      by the formation and the extent of rotten heart.
FAU - Peng, Hua-Sheng
AU  - Peng HS
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
AD  - State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese 
      Medical Sciences, Beijing, 100700 China. ISNI: 0000 0004 0632 3409. GRID: 
      grid.410318.f
FAU - Wang, Jun
AU  - Wang J
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
AD  - School of Pharmacy, Bozhou Vocational and Technical College, Bozhou, 236800 
      China.
FAU - Zhang, He-Ting
AU  - Zhang HT
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
FAU - Duan, Hai-Yan
AU  - Duan HY
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
FAU - Xie, Xiao-Mei
AU  - Xie XM
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
FAU - Zhang, Ling
AU  - Zhang L
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
FAU - Cheng, Ming-En
AU  - Cheng ME
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
AD  - Institute of TCM Resources Protection and Development, Anhui Academy of Chinese 
      Medicine, Hefei, 230031 People's Republic of China.
FAU - Peng, Dai-Yin
AU  - Peng DY
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031 China. 
      ISNI: 0000 0004 1757 8247. GRID: grid.252251.3
AD  - Institute of TCM Resources Protection and Development, Anhui Academy of Chinese 
      Medicine, Hefei, 230031 People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20170519
PL  - England
TA  - Chin Med
JT  - Chinese medicine
JID - 101265109
PMC - PMC5438511
OTO - NOTNLM
OT  - Concentration
OT  - Freehand section
OT  - Growth rings
OT  - Histochemical localization
OT  - Rotten heart
EDAT- 2017/05/24 06:00
MHDA- 2017/05/24 06:01
PMCR- 2017/05/19
CRDT- 2017/05/24 06:00
PHST- 2014/11/11 00:00 [received]
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/05/24 06:00 [entrez]
PHST- 2017/05/24 06:00 [pubmed]
PHST- 2017/05/24 06:01 [medline]
PHST- 2017/05/19 00:00 [pmc-release]
AID - 135 [pii]
AID - 10.1186/s13020-017-0135-z [doi]
PST - epublish
SO  - Chin Med. 2017 May 19;12:14. doi: 10.1186/s13020-017-0135-z. eCollection 2017.