PMID- 28534196
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2193-9616 (Print)
IS  - 2193-9616 (Electronic)
IS  - 2193-9616 (Linking)
VI  - 5
IP  - 1
DP  - 2016 Dec
TI  - In silico analysis of Shiga toxins (Stxs) to identify new potential vaccine 
      targets for Shiga toxin-producing Escherichia coli.
PG  - 2
LID - 10.1007/s40203-017-0022-4 [doi]
LID - 2
AB  - Shiga toxins belong to a family of structurally and functionally related toxins 
      serving as the main virulence factors for pathogenicity of the Shiga 
      toxin-producing Escherichia coli (STEC) associating with Hemolytic uremic 
      syndrome (HUS). At present, there is no effective treatment or prevention for 
      HUS. The aim of the present study was to find conserved regions within the amino 
      acid sequences of Stx1, Stx2 (Shiga toxin) and their variants. In this regard, 
      In-silico identification of conformational continuous B cell and T-cell epitopes 
      was performed in order to introduce new potential vaccine candidates. 93-100% 
      Homology was observed in Stx1 and its variants. In Stx2 and its variants, 69-100% 
      homology was shown. By sequence alignment with Stx1 and Stx2, 54% homology was 
      detected. T-cell epitope identification in Stx1A and Stx2A epitopes with highest 
      binding affinity for each HLA (human leukocyte antigen) was demonstrated with 
      100% identity among all Stxs. B-cell epitope prediction was resulted in finding 
      of four common epitopes between Stxs. In silico analysis of Stxs was resulted to 
      identification of new peptide targets that could be used in development of new 
      epitope vaccine candidates or in immunodiagnostic tests.
FAU - Golshani, Maryam
AU  - Golshani M
AD  - Molecular Biology Department, Institute Pasteur of Iran, Pasteur Ave., Tehran, 
      13164, Iran.
FAU - Oloomi, Mana
AU  - Oloomi M
AD  - Molecular Biology Department, Institute Pasteur of Iran, Pasteur Ave., Tehran, 
      13164, Iran. manaoloomi@yahoo.com.
FAU - Bouzari, Saeid
AU  - Bouzari S
AD  - Molecular Biology Department, Institute Pasteur of Iran, Pasteur Ave., Tehran, 
      13164, Iran.
LA  - eng
PT  - Journal Article
DEP - 20170522
PL  - Germany
TA  - In Silico Pharmacol
JT  - In silico pharmacology
JID - 101623954
PMC - PMC5440418
OTO - NOTNLM
OT  - B-cell epitope
OT  - EHEC
OT  - HUS
OT  - Protein analysis
OT  - T-cell epitope
OT  - Vaccine
COIS- The authors do not have any conflict of interest.
EDAT- 2017/05/24 06:00
MHDA- 2017/05/24 06:01
PMCR- 2017/05/22
CRDT- 2017/05/24 06:00
PHST- 2017/03/05 00:00 [received]
PHST- 2017/05/14 00:00 [accepted]
PHST- 2017/05/24 06:00 [entrez]
PHST- 2017/05/24 06:00 [pubmed]
PHST- 2017/05/24 06:01 [medline]
PHST- 2017/05/22 00:00 [pmc-release]
AID - 10.1007/s40203-017-0022-4 [pii]
AID - 22 [pii]
AID - 10.1007/s40203-017-0022-4 [doi]
PST - ppublish
SO  - In Silico Pharmacol. 2016 Dec;5(1):2. doi: 10.1007/s40203-017-0022-4. Epub 2017 
      May 22.