PMID- 28535452
OWN - NLM
STAT- MEDLINE
DCOM- 20180119
LR  - 20181202
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 142
DP  - 2017 Aug 5
TI  - Highly sensitive UHPLC-MS/MS method for the simultaneous estimation of 
      propafenone and its metabolites 5-hydroxypropafenone and N-depropylpropafenone on 
      human dried blood spots technique and application to a pharmacokinetic study.
PG  - 328-336
LID - S0731-7085(17)30851-8 [pii]
LID - 10.1016/j.jpba.2017.04.046 [doi]
AB  - A highly sensitive, rapid and selective UHPLC-MS/MS method has been developed and 
      validated for quantification of the propafenone (PF), 5-hydroxypropafenone 
      (5-OHPF) and N-depropylpropafenone (N-DPF) on human dried blood spot (DBS). The 
      assay procedure involves a solid-liquid extraction of PF, 5-OHPF and N-DPF and 
      amlodipine (internal standard, I.S.) from dried human DBS cards using water and 
      acetonitrile. The chromatographic resolution was achieved on a BEH C(18) column 
      using a gradient mobile phase consisting of 0.1% formic acid in water and 
      acetonitrile with 0.1% formic acid at flow rate of 0.6mL/min. The UHPLC-MS/MS was 
      operated under the multiple-reaction monitoring mode using electrospray 
      ionization. Total run time of analysis was 1.1min and elution of PF, 5-OHPF, 
      N-DPF and I.S. occurred at 0.69, 0.6, 0.68 and 0.73min, respectively. A detailed 
      method validation was performed as per the regulatory guidelines and the standard 
      curves found to be linear in the range of 5.11-1000ng/mL for PF and 5-OHPF and 
      0.51-100ng/mL for N-DPF with a correlation coefficient of >/=0.99 for all the 
      drugs. The intra- and inter-day accuracies were in the range of 95.6-107 and 
      93.5-103; 93.4-106 and 96.3-107 and 87.9-103 and 96.5-102%, for PF, 5-OHPF and 
      N-DPF, respectively. The intra- and inter-day precisions were in the range of 
      2.50-5.52 and 3.38-5.18; 2.16-6.34 and 3.23-4.94 and 2.63-7.55 and 1.56-10.2%, 
      for PF, 5-OHPF and N-DPF, respectively. The validated assay method was 
      successfully applied to a pharmacokinetic study in humans. The key 
      pharmacokinetic parameters AUC(0-infinity) and C(max) were 6057+/-1526, 2002+/-515 and 
      525+/-202 ng*h/mL and 653+/-183, 295+/-37.5 and 68.4+/-13.6ng/mL for PF, 5-OHPF and 
      N-DPF, respectively.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Andy, Adinarayana
AU  - Andy A
AD  - Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, 
      Guntur, 522510, Andhra Pradesh, India.
FAU - Kallem, Raja Reddy
AU  - Kallem RR
AD  - Science Office, School of Pharmacy, Texas Tech University Health Sciences Center, 
      Amarillo, TX, 79106, United States.
FAU - Mullangi, Ramesh
AU  - Mullangi R
AD  - Jubilant Biosys, 2nd Stage, Industrial Suburb, Yeshwanthpur, Bangalore, 560 022, 
      India. Electronic address: mullangi_ramesh@jubilantinnovation.com.
FAU - Andy, Divya
AU  - Andy D
AD  - Department of Medicinal and Biological Chemistry, College of Pharmacy and 
      Pharmaceutical Sciences, University of Toledo, 3000 Arlington Avenue, Toledo OH 
      43614, United States.
FAU - Seshagiri Rao, J V L N
AU  - Seshagiri Rao JVLN
AD  - Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, 
      Guntur, 522510, Andhra Pradesh, India; College of Pharmaceutical Sciences, Andhra 
      University, Visakhapatnam, 530 003, Andhra Pradesh, India.
LA  - eng
PT  - Journal Article
DEP - 20170511
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 68IQX3T69U (Propafenone)
RN  - 7AC8BF38NP (5-hydroxypropafenone)
RN  - KN087PHW5T (N-depropylpropafenone)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - *Dried Blood Spot Testing
MH  - Humans
MH  - Propafenone/analogs & derivatives
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - 5-hydroxypropafenone
OT  - Bioanalysis
OT  - DBS
OT  - Human blood
OT  - LC-MS/MS
OT  - Method validation
OT  - N-depropylpropafenone
OT  - Pharmacokinetics
OT  - Propafenone
EDAT- 2017/05/24 06:00
MHDA- 2018/01/20 06:00
CRDT- 2017/05/24 06:00
PHST- 2017/04/05 00:00 [received]
PHST- 2017/04/25 00:00 [revised]
PHST- 2017/04/28 00:00 [accepted]
PHST- 2017/05/24 06:00 [pubmed]
PHST- 2018/01/20 06:00 [medline]
PHST- 2017/05/24 06:00 [entrez]
AID - S0731-7085(17)30851-8 [pii]
AID - 10.1016/j.jpba.2017.04.046 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2017 Aug 5;142:328-336. doi: 10.1016/j.jpba.2017.04.046. 
      Epub 2017 May 11.