PMID- 2853691 OWN - NLM STAT- MEDLINE DCOM- 19890519 LR - 20191210 IS - 0378-1119 (Print) IS - 0378-1119 (Linking) VI - 70 IP - 1 DP - 1988 Oct 15 TI - A comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter. PG - 51-6 AB - Three transcriptional regulatory regions including a human cytomegalovirus immediate-early region (CMVIE) promoter, the simian virus-40 early region (SV40E) promoter, and Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) were ligated to the bovine growth hormone (bGH)-coding gene. Using a bGH transient expression system, the CMVIE and SV40E gene transcriptional regulatory regions were found to be approximately two-fold more efficient than the MoMLV LTR in directing expression of bGH in mouse L cells. FAU - Martin-Gallardo, A AU - Martin-Gallardo A AD - Department of Zoology and Biomedical Sciences, Edison Animal Biotechnology Center, Athens, OH. FAU - Montoya-Zavala, M AU - Montoya-Zavala M FAU - Kelder, B AU - Kelder B FAU - Taylor, J AU - Taylor J FAU - Chen, H AU - Chen H FAU - Leung, F C AU - Leung FC FAU - Kopchick, J J AU - Kopchick JJ LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Gene JT - Gene JID - 7706761 RN - 0 (DNA, Recombinant) RN - 9002-72-6 (Growth Hormone) SB - IM MH - Animals MH - Cattle MH - Cytomegalovirus/genetics MH - DNA, Recombinant MH - *Genes, Regulator MH - Genetic Vectors MH - Growth Hormone/*genetics MH - L Cells MH - Mice MH - Moloney murine leukemia virus/genetics MH - Plasmids MH - *Promoter Regions, Genetic MH - Simian virus 40/genetics MH - Transfection EDAT- 1988/10/15 00:00 MHDA- 1988/10/15 00:01 CRDT- 1988/10/15 00:00 PHST- 1988/10/15 00:00 [pubmed] PHST- 1988/10/15 00:01 [medline] PHST- 1988/10/15 00:00 [entrez] AID - 0378-1119(88)90103-5 [pii] AID - 10.1016/0378-1119(88)90103-5 [doi] PST - ppublish SO - Gene. 1988 Oct 15;70(1):51-6. doi: 10.1016/0378-1119(88)90103-5.