PMID- 28539717
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 13
IP  - 50
DP  - 2017 Apr-Jun
TI  - Caspase-mediated Apoptotic Effects of Ebenus boissieri Barbey Extracts on Human 
      Cervical Cancer Cell Line HeLa.
PG  - 254-259
LID - 10.4103/0973-1296.204560 [doi]
AB  - BACKGROUND: Ebenus boissieri Barbey is an Antalya, Turkey-endemic plant belonging 
      to Fabaceae family. The aerial parts and the roots of E. boissieri Barbey were 
      used in this study. OBJECTIVE: In the present study, we have examined the 
      apoptotic effects of hydroalcoholic extracts of E. boissieri Barbey in human 
      cervical cancer cell line HeLa. MATERIALS AND METHODS: To determine the cytotoxic 
      effect, cells were treated with various concentrations of extracts for 24, 48, 
      and 72 h incubation periods. Cytotoxic effects were examined by Cell Titer 96 
      aqueous nonradioactive cell proliferation assay and the results were corrected by 
      live/dead viability/cytotoxicity assay and trypan blue exclusion assay. Apoptotic 
      effects were studied with multicaspase kit. Tumor necrosis factor-alpha (TNF-alpha) 
      and interferon gamma (IFN-gamma) release were also measured by enzyme-linked 
      immunosorbent assay. RESULTS: According to the results, E. boissieri Barbey 
      extract caused significant increase in caspase levels. Thus, we suggest that the 
      extract induces cells to undergo apoptosis. Especially, there was a sharp 
      induction in caspase-3 activity. Levels of both TNF-alpha and IFN-gamma in 
      extract-treated groups were significantly and dose dependently exalted as 
      compared to their relative controls. CONCLUSION: The effects of the extract on 
      caspase-3, TNF-alpha, and IFN-gamma levels mediate the plausible mechanism of apoptosis 
      induction in HeLa. To the best of our knowledge, this is the first report 
      indicating any pharmacological properties of E. Boissieri on HeLa cells. SUMMARY: 
      HeLa cell viability was reduced in dose-dependent manner for 72 h with an IC50 of 
      approximate 28.03 mug/mL for aerial and 41.02 mug/mL for rootHeLa cells, exposure 
      to the aerial extract led to 1.9, 3.8, 1.2, 2.4, and 3.45 fold induction of all 
      caspases activities (-2, -3, -6, -8, and -9, respectively)Both 30 mug/mL of aerial 
      and 45 mug/mL of root extracts allowed the production of anticancer cytokines 
      (TNFalpha; IFNgamma) in HeLa cell culture supernatants. Abbreviations used: Tumor 
      necrosis factor-alpha (TNF-alpha); Interferon gamma (IFN-gamma); 3-(4, 5 
      dimethylthiazol-2-yl)-5-(3- carboxymethoxy-phenyl)-2-(4-sulfonyl)-2H-tetrazolium 
      (MTS); Phosphate-Buffered Saline (PBS); Fetal Bovine Serum (FBS); 
      para-Nitroanilin pNA; Enzyme-Linked ImmunoSorbent Assay (ELISA); Sodium Dodesyl 
      sulphate -Polyacrilamide gel electrophoresis (SDS-PAGE); Tris-Buffered Saline 
      (TBS); Hydocloric acid (HCl); Standart Error of Mean (SEM); National Cancer 
      Institute (NCI); half maximal inhibitory concentration (IC(50)).
FAU - Simsek, Ece
AU  - Simsek E
AD  - Department of Nutrition and Dietetics, Antalya School of Health, Akdeniz 
      University, Akdeniz, Antalya, Turkey.
FAU - Imir, Nilufer
AU  - Imir N
AD  - Department of Biology Education, Faculty of Education and Institute of Life 
      Sciences, Akdeniz University, Akdeniz, Antalya, Turkey.
FAU - Aydemir, Esra Arslan
AU  - Aydemir EA
AD  - Department of Biology, Faculty of Sciences, Akdeniz University, Akdeniz, Antalya, 
      Turkey.
FAU - Gokturk, Ramazan Suleyman
AU  - Gokturk RS
AD  - Department of Biology, Faculty of Sciences, Akdeniz University, Akdeniz, Antalya, 
      Turkey.
FAU - Yesilada, Erdem
AU  - Yesilada E
AD  - Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Atasehir, 
      Istanbul, Turkey.
FAU - Fiskin, Kayahan
AU  - Fiskin K
AD  - Department of Biology, Faculty of Sciences, Akdeniz University, Akdeniz, Antalya, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20170418
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC5421422
OTO - NOTNLM
OT  - Apoptosis
OT  - Ebenus
OT  - caspase
OT  - cervical cancer
OT  - interferon-gamma
OT  - tumor necrosis factor
COIS- There are no conflicts of interest
EDAT- 2017/05/26 06:00
MHDA- 2017/05/26 06:01
PMCR- 2017/04/01
CRDT- 2017/05/26 06:00
PHST- 2015/08/07 00:00 [received]
PHST- 2015/09/29 00:00 [revised]
PHST- 2017/05/26 06:00 [entrez]
PHST- 2017/05/26 06:00 [pubmed]
PHST- 2017/05/26 06:01 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - PM-13-254 [pii]
AID - 10.4103/0973-1296.204560 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2017 Apr-Jun;13(50):254-259. doi: 10.4103/0973-1296.204560. Epub 
      2017 Apr 18.