PMID- 28542228
OWN - NLM
STAT- MEDLINE
DCOM- 20170919
LR  - 20190208
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 5
DP  - 2017
TI  - Alteration of membrane complement regulators is associated with transporter 
      status in patients on peritoneal dialysis.
PG  - e0177487
LID - 10.1371/journal.pone.0177487 [doi]
LID - e0177487
AB  - INTRODUCTION: A growing body of evidence from animal models and cell culture 
      studies indicate an important role of a local regulatory complement system (CS) 
      in peritoneal injury during peritoneal dialysis (PD). We investigated the 
      expression of the local regulatory CS (reflected by CD46,CD55,CD59) in the 
      peritoneal tissue of patients with different membrane function characteristics. 
      PATIENTS AND METHODS: Biopsies from the parietal peritoneum were taken from 24 
      patients on PD, 22 uremic patients prior to PD. PD patients were grouped 
      according to the dialysate-to-plasma ratio of creatinine (D/P Cre) and ratio of 
      dialysate glucose at 4 hours versus dialysate glucose at time zero (D/D0 glucose) 
      into low or low-average peritoneal transport status (L/LA) and high-average or 
      high-transport status (HA/H) groups. CD46, CD55, and CD59 RNA expression were 
      analyzed by real-time polymerase chain reaction (RT-PCR). Further localization of 
      membrane complement regulators (CRegs) and semiquantitatively analysis was done 
      by immunohistochemistry (IHC). RESULTS: CD46 and CD59 expression were similar in 
      all groups. CD55 expression was significantly decreased in the HA/H group 
      compared to the L/LA group and to uremic controls (p < 0.05 and p = 0.05, 
      respectively). No statistically significant differences in CD46, CD55, and CD55 
      expression were detected when considering the history of peritonitis. There was 
      no statistically significant correlation between PD duration and the expressions 
      of CD46, CD55, and CD59. IHC revealed strong CD46, CD55, and CD59 expression in 
      mesothelial cells. CD55 and CD59 were additionally detected in the vasculature. 
      Using IHC, CD46 was lower in PD patients compared to uremic controls (p>0.05), 
      but there was no difference between the L/LA compared to the H/HA group. Moreover 
      IHC confirmed decreased expression of CD55 in the HA/H group compared to the L/LA 
      group and uremic controls (p<0.0001 and p = 0.0001, respectively). CONCLUSION: 
      CD55 expression is decreased in patients with fast transporter membrane function, 
      whereas peritonitis and PD duration do not appear to alter CReg expression.
FAU - Kitterer, Daniel
AU  - Kitterer D
AUID- ORCID: 0000-0002-6532-823X
AD  - Department of Internal Medicine, Division of Nephrology, Robert-Bosch-Hospital, 
      Stuttgart, Germany.
FAU - Biegger, Dagmar
AU  - Biegger D
AD  - Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of 
      Tuebingen, Stuttgart, Germany.
FAU - Segerer, Stephan
AU  - Segerer S
AD  - Division of Nephrology, Dialysis & Transplantation, Kantonsspital Aarau, Aarau, 
      Switzerland.
FAU - Braun, Niko
AU  - Braun N
AD  - Nephrology Center Stuttgart, Stuttgart, Germany.
FAU - Alscher, M Dominik
AU  - Alscher MD
AD  - Department of Internal Medicine, Division of Nephrology, Robert-Bosch-Hospital, 
      Stuttgart, Germany.
FAU - Latus, Joerg
AU  - Latus J
AD  - Department of Internal Medicine, Division of Nephrology, Robert-Bosch-Hospital, 
      Stuttgart, Germany.
LA  - eng
PT  - Journal Article
DEP - 20170519
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, CD)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antigens, CD/genetics/*metabolism
MH  - Cell Membrane/*metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Membrane Transport Proteins/genetics/*metabolism
MH  - Middle Aged
MH  - *Peritoneal Dialysis
MH  - Peritoneum/cytology
MH  - Peritonitis/metabolism
MH  - RNA, Messenger/genetics/metabolism
PMC - PMC5438122
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/05/26 06:00
MHDA- 2017/09/20 06:00
PMCR- 2017/05/19
CRDT- 2017/05/26 06:00
PHST- 2017/01/30 00:00 [received]
PHST- 2017/04/27 00:00 [accepted]
PHST- 2017/05/26 06:00 [entrez]
PHST- 2017/05/26 06:00 [pubmed]
PHST- 2017/09/20 06:00 [medline]
PHST- 2017/05/19 00:00 [pmc-release]
AID - PONE-D-17-03853 [pii]
AID - 10.1371/journal.pone.0177487 [doi]
PST - epublish
SO  - PLoS One. 2017 May 19;12(5):e0177487. doi: 10.1371/journal.pone.0177487. 
      eCollection 2017.