PMID- 28543841
OWN - NLM
STAT- MEDLINE
DCOM- 20180713
LR  - 20180713
IS  - 1440-1746 (Electronic)
IS  - 0815-9319 (Linking)
VI  - 33
IP  - 1
DP  - 2018 Jan
TI  - Role of fluorescence in situ hybridization in diagnosing cholangiocarcinoma in 
      indeterminate biliary strictures.
PG  - 315-319
LID - 10.1111/jgh.13824 [doi]
AB  - BACKGROUND AND AIM: Brush cytology, the conventional method to diagnose 
      cholangiocarcinoma, has been plagued by low diagnostic sensitivity and 
      false-negative results. This paper aims to study the clinical utility of 
      fluorescence in situ hybridization (FISH) in enhancing identification of 
      malignant biliary strictures. METHODS: Brush cytologic specimens collected from 
      endoscopic retrograde cholangiopancreatography for biliary strictures in a 
      tertiary hospital in Singapore from March 2013 to July 2015 were examined by FISH 
      technique using UroVysion probe set in this study. RESULTS: Thirty patients were 
      chosen with five patients having multiple FISH performed due to indeterminate 
      results. The diagnoses for biliary strictures were 13 (43.3%) 
      cholangiocarcinomas, seven (23.3%) pancreatic cancers, seven (23.3%) benign 
      biliary strictures, and three (10%) primary sclerosing cholangitis. Conventional 
      brush cytology had sensitivity of 53.8% with specificity of 82.4%. FISH had 
      sensitivity of 30.8% with specificity of 100%. When FISH results were interpreted 
      in cases with negative or atypical brush cytology, two patients had positive FISH 
      results and cholangiocarcinomas. Based on this pilot study, FISH increased 
      sensitivity of brush cytology in detection of cholangiocarcinoma from 53.8% to 
      69.2% while preserving specificity of 82.4%. CONCLUSION: Compared with 
      conventional cytology with low sensitivity, FISH may help to increase sensitivity 
      on top of brush cytology while maintaining high specificity.
CI  - (c) 2017 Journal of Gastroenterology and Hepatology Foundation and John Wiley & 
      Sons Australia, Ltd.
FAU - Liew, Zhong Hong
AU  - Liew ZH
AUID- ORCID: 0000-0002-7943-1510
AD  - Department of Gastroenterology and Hepatology, Singapore General Hospital, 
      Singapore.
FAU - Loh, Tracy Jiezhen
AU  - Loh TJ
AD  - Department of Pathology, Singapore General Hospital, Singapore.
FAU - Lim, Tony Kiat Hon
AU  - Lim TKH
AD  - Department of Pathology, Singapore General Hospital, Singapore.
FAU - Lim, Tse Hui
AU  - Lim TH
AD  - Department of Pathology, Singapore General Hospital, Singapore.
FAU - Khor, Christopher Jen Lock
AU  - Khor CJL
AD  - Department of Gastroenterology and Hepatology, Singapore General Hospital, 
      Singapore.
FAU - Mesenas, Steven Joseph
AU  - Mesenas SJ
AD  - Department of Gastroenterology and Hepatology, Singapore General Hospital, 
      Singapore.
FAU - Kong, Chris San Choon
AU  - Kong CSC
AD  - Department of Gastroenterology and Hepatology, Singapore General Hospital, 
      Singapore.
FAU - Ong, Wai Choung
AU  - Ong WC
AD  - Department of Gastroenterology and Hepatology, Singapore General Hospital, 
      Singapore.
FAU - Tan, Damien Meng Yew
AU  - Tan DMY
AD  - Department of Gastroenterology and Hepatology, Singapore General Hospital, 
      Singapore.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - J Gastroenterol Hepatol
JT  - Journal of gastroenterology and hepatology
JID - 8607909
SB  - IM
MH  - Aged
MH  - Bile Duct Neoplasms/*diagnosis/*pathology
MH  - Bile Ducts/*pathology
MH  - Cholangiocarcinoma/*diagnosis/*pathology
MH  - Cholangiopancreatography, Endoscopic Retrograde
MH  - Constriction, Pathologic
MH  - Cytological Techniques/*methods
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pilot Projects
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - cholangiocarcinoma
OT  - fluorescence in situ hybridization
OT  - indeterminate biliary stricture
EDAT- 2017/05/26 06:00
MHDA- 2018/07/14 06:00
CRDT- 2017/05/26 06:00
PHST- 2017/01/25 00:00 [received]
PHST- 2017/05/10 00:00 [revised]
PHST- 2017/05/13 00:00 [accepted]
PHST- 2017/05/26 06:00 [pubmed]
PHST- 2018/07/14 06:00 [medline]
PHST- 2017/05/26 06:00 [entrez]
AID - 10.1111/jgh.13824 [doi]
PST - ppublish
SO  - J Gastroenterol Hepatol. 2018 Jan;33(1):315-319. doi: 10.1111/jgh.13824.