PMID- 28545392
OWN - NLM
STAT- MEDLINE
DCOM- 20180511
LR  - 20181113
IS  - 1471-2121 (Electronic)
IS  - 1471-2121 (Linking)
VI  - 18
IP  - 1
DP  - 2017 May 25
TI  - Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells 
      during chemotaxis.
PG  - 23
LID - 10.1186/s12860-017-0139-7 [doi]
LID - 23
AB  - BACKGROUND: In Dictyostelium discoideum, vesicular transport of the adenylyl 
      cyclase A (ACA) to the posterior of polarized cells is essential to relay 
      exogenous 3',5'-cyclic adenosine monophosphate (cAMP) signals during chemotaxis 
      and for the collective migration of cells in head-to-tail arrangements called 
      streams. RESULTS: Using fluorescence in situ hybridization (FISH), we discovered 
      that the ACA mRNA is asymmetrically distributed at the posterior of polarized 
      cells. Using both standard estimators and Monte Carlo simulation methods, we 
      found that the ACA mRNA enrichment depends on the position of the cell within a 
      stream, with the posterior localization of ACA mRNA being strongest for cells at 
      the end of a stream. By monitoring the recovery of ACA-YFP after cycloheximide 
      (CHX) treatment, we observed that ACA mRNA and newly synthesized ACA-YFP first 
      emerge as fluorescent punctae that later accumulate to the posterior of cells. We 
      also found that the ACA mRNA localization requires 3' ACA cis-acting elements. 
      CONCLUSIONS: Together, our findings suggest that the asymmetric distribution of 
      ACA mRNA allows the local translation and accumulation of ACA protein at the 
      posterior of cells. These data represent a novel functional role for localized 
      translation in the relay of chemotactic signal during chemotaxis.
FAU - Das, Satarupa
AU  - Das S
AD  - Laboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 
      Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.
AD  - Institute for Physical Science and Technology, Department of Physics, University 
      of Maryland, College Park, MD, 20742, USA.
FAU - Parker, Joshua M
AU  - Parker JM
AD  - Institute for Physical Science and Technology, Department of Physics, University 
      of Maryland, College Park, MD, 20742, USA.
FAU - Guven, Can
AU  - Guven C
AD  - Institute for Physical Science and Technology, Department of Physics, University 
      of Maryland, College Park, MD, 20742, USA.
FAU - Wang, Weiye
AU  - Wang W
AD  - Laboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 
      Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.
FAU - Kriebel, Paul W
AU  - Kriebel PW
AD  - Laboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 
      Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.
FAU - Losert, Wolfgang
AU  - Losert W
AD  - Institute for Physical Science and Technology, Department of Physics, University 
      of Maryland, College Park, MD, 20742, USA.
FAU - Larson, Daniel R
AU  - Larson DR
AD  - Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, 
      NCI, NIH, Bethesda, MD, 20892, USA.
FAU - Parent, Carole A
AU  - Parent CA
AUID- ORCID: 0000-0002-1518-1452
AD  - Laboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 
      Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA. 
      parentc@mail.nih.gov.
AD  - Institute for Physical Science and Technology, Department of Physics, University 
      of Maryland, College Park, MD, 20742, USA. parentc@mail.nih.gov.
LA  - eng
GR  - R01 GM085574/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170525
PL  - England
TA  - BMC Cell Biol
JT  - BMC cell biology
JID - 100966972
RN  - 0 (Protozoan Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Protozoan)
RN  - 0 (Regulatory Sequences, Ribonucleic Acid)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - *Adenylyl Cyclases/genetics/metabolism
MH  - Animals
MH  - Cell Polarity/drug effects/genetics
MH  - Cells, Cultured
MH  - Chemotaxis/drug effects/*genetics
MH  - Cycloheximide/pharmacology
MH  - Cytoplasm/enzymology
MH  - Cytoplasmic Streaming/drug effects/physiology
MH  - Dictyostelium/*enzymology/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Protein Biosynthesis/drug effects
MH  - *Protozoan Proteins/genetics/metabolism
MH  - RNA Transport/physiology
MH  - RNA, Messenger/analysis/*genetics/*metabolism
MH  - RNA, Protozoan/analysis/genetics/metabolism
MH  - Regulatory Sequences, Ribonucleic Acid/physiology
MH  - Signal Transduction
PMC - PMC5445419
OTO - NOTNLM
OT  - Chemotaxis
OT  - Dictyostelium
OT  - Signal relay
OT  - mRNA
EDAT- 2017/05/27 06:00
MHDA- 2018/05/12 06:00
PMCR- 2017/05/25
CRDT- 2017/05/27 06:00
PHST- 2017/02/21 00:00 [received]
PHST- 2017/05/09 00:00 [accepted]
PHST- 2017/05/27 06:00 [entrez]
PHST- 2017/05/27 06:00 [pubmed]
PHST- 2018/05/12 06:00 [medline]
PHST- 2017/05/25 00:00 [pmc-release]
AID - 10.1186/s12860-017-0139-7 [pii]
AID - 139 [pii]
AID - 10.1186/s12860-017-0139-7 [doi]
PST - epublish
SO  - BMC Cell Biol. 2017 May 25;18(1):23. doi: 10.1186/s12860-017-0139-7.