PMID- 28545480
OWN - NLM
STAT- MEDLINE
DCOM- 20180102
LR  - 20181113
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 17
IP  - 1
DP  - 2017 May 25
TI  - Effects of hepatitis C virus core protein and nonstructural protein 4B on the 
      Wnt/beta-catenin pathway.
PG  - 124
LID - 10.1186/s12866-017-1032-4 [doi]
LID - 124
AB  - BACKGROUND: Hepatitis C virus (HCV) core protein and nonstructural protein 4B 
      (NS4B) are potentially oncogenic. Aberrant activation of the Wnt/beta-catenin 
      signaling pathway is closely associated with hepatocarcinogenesis. We 
      investigated the effects of HCV type 1b core protein and NS4B on Wnt/beta-catenin 
      signaling in various liver cells, and explored the molecular mechanism underlying 
      HCV-related hepatocarcinogenesis. RESULTS: Compared with the empty vector 
      control, HCV core protein and NS4B demonstrated the following characteristics in 
      the Huh7 cells: significantly enhanced beta-catenin/Tcf-dependent transcriptional 
      activity (F = 40.87, P < 0.01); increased nuclear translocation of beta-catenin 
      (F = 165.26, P < 0.01); upregulated nuclear beta-catenin, cytoplasmic beta-catenin, 
      Wnt1, c-myc, and cyclin D1 protein expression (P < 0.01); and promoted 
      proliferation of Huh7 cells (P < 0.01 or P < 0.05). Neither protein enhanced 
      beta-catenin/Tcf-dependent transcriptional activity in the LO2 cells (F = 0.65, 
      P > 0.05), but they did significantly enhance Wnt3a-induced 
      beta-catenin/Tcf-dependent transcriptional activity (F = 64.25, P < 0.01), and 
      promoted the nuclear translocation of beta-catenin (F = 66.54, P < 0.01) and the 
      Wnt3a-induced proliferation of LO2 cells (P < 0.01 or P < 0.05). Moreover, 
      activation of the Wnt/beta-catenin signaling pathway was greater with the core 
      protein than with NS4B (P < 0.01 or P < 0.05). CONCLUSIONS: HCV core protein and 
      NS4B directly activate the Wnt/beta-catenin signaling pathway in Huh7 cells and LO2 
      cells induced by Wnt3a. These data suggest that HCV core protein and NS4B 
      contribute to HCV-associated hepatocellular carcinogenesis.
FAU - Jiang, Xiao-Hua
AU  - Jiang XH
AD  - Department of Infectious Diseases, the First Affiliated Hospital of the 
      University of South China, Hengyang, 421001, China.
FAU - Xie, Yu-Tao
AU  - Xie YT
AUID- ORCID: 0000-0003-1634-2059
AD  - Department of Infectious Diseases, Xiangya Hospital of Central South University, 
      Changsha, 410087, China. yutaoxiedoc@126.com.
FAU - Cai, Ya-Ping
AU  - Cai YP
AD  - Department of Epidemiology and Health Statistics, the University of South China, 
      Hengyang, 421001, China.
FAU - Ren, Jing
AU  - Ren J
AD  - Department of Infectious Diseases, the First Affiliated Hospital of the 
      University of South China, Hengyang, 421001, China.
FAU - Ma, Tao
AU  - Ma T
AD  - Department of Infectious Diseases, the First Affiliated Hospital of the 
      University of South China, Hengyang, 421001, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170525
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
RN  - 0 (CCND1 protein, human)
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (NS4B protein, flavivirus)
RN  - 0 (Viral Core Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (beta Catenin)
RN  - 0 (nucleocapsid protein, Hepatitis C virus)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Animals
MH  - Cell Cycle/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cyclin D1/metabolism
MH  - Hepatitis C/*metabolism
MH  - Humans
MH  - Mice
MH  - Signal Transduction/drug effects
MH  - Transcription, Genetic
MH  - Viral Core Proteins/*pharmacology
MH  - Viral Nonstructural Proteins/*pharmacology
MH  - Wnt Signaling Pathway/*drug effects
MH  - beta Catenin/*drug effects/metabolism
PMC - PMC5445264
OTO - NOTNLM
OT  - Core protein
OT  - HCV
OT  - NS4B
OT  - Wnt/beta-catenin signaling pathway
EDAT- 2017/05/27 06:00
MHDA- 2018/01/03 06:00
PMCR- 2017/05/25
CRDT- 2017/05/27 06:00
PHST- 2017/01/13 00:00 [received]
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/05/27 06:00 [entrez]
PHST- 2017/05/27 06:00 [pubmed]
PHST- 2018/01/03 06:00 [medline]
PHST- 2017/05/25 00:00 [pmc-release]
AID - 10.1186/s12866-017-1032-4 [pii]
AID - 1032 [pii]
AID - 10.1186/s12866-017-1032-4 [doi]
PST - epublish
SO  - BMC Microbiol. 2017 May 25;17(1):124. doi: 10.1186/s12866-017-1032-4.