PMID- 28546857
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2045-3701 (Print)
IS  - 2045-3701 (Electronic)
IS  - 2045-3701 (Linking)
VI  - 7
DP  - 2017
TI  - Chloroquine inhibits Ca(2+) permeable ion channels-mediated Ca(2+) signaling in 
      primary B lymphocytes.
PG  - 28
LID - 10.1186/s13578-017-0155-5 [doi]
LID - 28
AB  - BACKGROUND: Chloroquine, a bitter tastant, inhibits Ca(2+) signaling, resulting 
      in suppression of B cell activation; however, the inhibitory mechanism remains 
      unclear. RESULTS: In this study, thapsigargin (TG), but not caffeine, induced 
      sustained intracellular Ca(2+) increases in mouse splenic primary B lymphocytes, 
      which were markedly inhibited by chloroquine. Under Ca(2+)-free conditions, TG 
      elicited transient Ca(2+) increases, which additionally elevated upon the 
      restoration of 2 mM Ca(2+). The former were from release of intracellular Ca(2+) 
      store and the latter from Ca(2+) influx. TG-induced release was inhibited by 
      2-APB (an inhibitor of inositol-3-phosphate receptors, IP(3)Rs) and chloroquine, 
      and TG-caused influx was inhibited by pyrazole (Pyr3, an inhibitor of transient 
      receptor potential C3 (TRPC3) and stromal interaction molecule (STIM)/Orai 
      channels) and chloroquine. Moreover, chloroquine also blocked Ca(2+) increases 
      induced by the engagement of B cell receptor (BCR) with anti-IgM. CONCLUSIONS: 
      These results indicate that chloroquine inhibits Ca(2+) elevations in splenic B 
      cells through inhibiting Ca(2+) permeable IP(3)R and TRPC3 and/or STIM/Orai 
      channels. These findings suggest that chloroquine would be a potent 
      immunosuppressant.
FAU - Wu, Yi-Fan
AU  - Wu YF
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Zhao, Ping
AU  - Zhao P
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Luo, Xi
AU  - Luo X
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Xu, Jin-Chao
AU  - Xu JC
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Xue, Lu
AU  - Xue L
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Zhou, Qi
AU  - Zhou Q
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Xiong, Mingrui
AU  - Xiong M
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Shen, Jinhua
AU  - Shen J
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Peng, Yong-Bo
AU  - Peng YB
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Yu, Meng-Fei
AU  - Yu MF
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Chen, Weiwei
AU  - Chen W
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Ma, Liqun
AU  - Ma L
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
FAU - Liu, Qing-Hua
AU  - Liu QH
AD  - Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection 
      and Application of Special Plants in Wuling Area of China, College of Life 
      Sciences, South Central University for Nationalities, Wuhan, 430074 China. ISNI: 
      0000 0000 9147 9053. GRID: grid.412692.a
LA  - eng
PT  - Journal Article
DEP - 20170523
PL  - England
TA  - Cell Biosci
JT  - Cell & bioscience
JID - 101561195
PMC - PMC5442594
OTO - NOTNLM
OT  - B cells
OT  - Ca2+
OT  - Chloroquine
OT  - IP3R
OT  - STIM/Orai channels
OT  - TRPC3 channels
EDAT- 2017/05/27 06:00
MHDA- 2017/05/27 06:01
PMCR- 2017/05/23
CRDT- 2017/05/27 06:00
PHST- 2016/12/30 00:00 [received]
PHST- 2017/05/19 00:00 [accepted]
PHST- 2017/05/27 06:00 [entrez]
PHST- 2017/05/27 06:00 [pubmed]
PHST- 2017/05/27 06:01 [medline]
PHST- 2017/05/23 00:00 [pmc-release]
AID - 155 [pii]
AID - 10.1186/s13578-017-0155-5 [doi]
PST - epublish
SO  - Cell Biosci. 2017 May 23;7:28. doi: 10.1186/s13578-017-0155-5. eCollection 2017.