PMID- 28547959
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20170726
IS  - 1233-9687 (Print)
IS  - 1233-9687 (Linking)
VI  - 67
IP  - 4
DP  - 2016
TI  - [Tissue heterogeneity contributes to suboptimal precision of WHO 2010 scoring 
      criteria for Ki67 labeling index in a subset of neuroendocrine neoplasms of the 
      pancreas].
PG  - 318-331
LID - 29474 [pii]
LID - 10.5114/pjp.2016.65864 [doi]
AB  - Reporting of Ki67 labeling index (LI) is a routine in diagnostics of 
      neuroendocrine neoplasms of the pancreas. The aim of the study was to examine 
      whether heterogeneity of Ki67 LI distribution in primary tumoral tissue 
      influences precision of reporting of Ki67 LI and Ki67-LI-based grade, both 
      established in adherence to WHO 2010 guidelines. Seventy-one samples of 
      neuroendocrine tumours (NET) and 6 samples of neuroendocrine carcinomas (NEC) of 
      the pancreas were taken for manual counting of Ki67 LI in 25 portions of 100 
      cells (2500 cells in total) in 3 hot spots an in a single area of lower 
      proliferation rate (cold spot) in each case. Both NET and NEC showed Ki67 LI 
      heterogeneity within primary tumour. Almost 20% of NET showed higher grade when 
      500 cells rather than 2000 cells were counted in hot spot area. Suboptimal choice 
      of hot spot resulted in under-grading of approximately 20% of NET. Cold spots 
      were constantly present in NET. Heterogeneity of Ki67 LI was also present in NEC, 
      but it virtually never resulted in under-grading. Concept and methodology of Ki67 
      LI counting in neuroendocrine neoplasms of the pancreas requires clarification. 
      Efforts aiming to improve precision of assessment of Ki67 LI are needed.
FAU - Liszka, Lukasz
AU  - Liszka L
LA  - pol
PT  - Journal Article
PL  - Poland
TA  - Pol J Pathol
JT  - Polish journal of pathology : official journal of the Polish Society of 
      Pathologists
JID - 9437432
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Humans
MH  - Immunohistochemistry
MH  - Ki-67 Antigen/*analysis
MH  - Mitotic Index/*methods
MH  - Neoplasm Grading/*methods
MH  - Neuroendocrine Tumors/*pathology
MH  - World Health Organization
OTO - NOTNLM
OT  - neoplasm grading
OT  - neuroendocrine tumors
OT  - neuroendo-crine carcinoma
OT  - pancreas
OT  - pancreatic neoplasms
OT  - Ki-67 antigen
EDAT- 2016/01/01 00:00
MHDA- 2017/07/27 06:00
CRDT- 2017/05/27 06:00
PHST- 2017/05/27 06:00 [entrez]
PHST- 2016/01/01 00:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
AID - 29474 [pii]
AID - 10.5114/pjp.2016.65864 [doi]
PST - ppublish
SO  - Pol J Pathol. 2016;67(4):318-331. doi: 10.5114/pjp.2016.65864.