PMID- 28549801
OWN - NLM
STAT- MEDLINE
DCOM- 20180516
LR  - 20180925
IS  - 1098-8823 (Print)
IS  - 1098-8823 (Linking)
VI  - 131
DP  - 2017 Jul
TI  - Methanolic extract of Boswellia serrata exhibits anti-cancer activities by 
      targeting microsomal prostaglandin E synthase-1 in human colon cancer cells.
PG  - 1-8
LID - S1098-8823(16)30125-3 [pii]
LID - 10.1016/j.prostaglandins.2017.05.003 [doi]
AB  - BACKGROUND: Colorectal cancer (CRC) is the most common cancer. A proper method to 
      reduce mortality of CRC is chemoprevention to prevent initiation and promotion of 
      intestinal tumorgenesis. One of the promising and developing chemopreventive 
      agents is natural compounds found in plants. Frankincense, the resin extract from 
      the Boswellia specious, has been used in traditional and modern medicine for 
      treating various diseases with very minimal side effects. In the current study, 
      we investigated the anti-cancer activity of methanolic extract of Boswellia 
      serrata (B. serrata) on HT-29 human colon cancer cells. METHODS: HT-29 cells were 
      treated with different concentrations of B. serrata and cell viability was 
      assessed by MTT assay. mRNA expression of microsomal prostaglandin E synthase-1 
      (mPGES-1), vascular endothelial growth factor (VEGF), C-X-C chemokine receptor 
      type 4 (CXCR4), matrix metalloproteinase-2 (MMP-2), MMP-9 and hypoxia-inducible 
      factor-1 (HIF-1) were examined by quantitative real-time PCR. Apoptosis was 
      evaluated by the proportion of sub-G1 cells. Prostaglandin E2 (PGE2) level and 
      caspase 3 activity were determined by ELISA assay. Tube formation potential and 
      HT-29 cells migration were assessed using three-dimensional vessel formation 
      assay and scratch test. RESULTS: B. serrata extract considerably decreased the 
      expression of mPGES-1, VEGF, CXCR4, MMP-2, MMP-9 and HIF-1. The caspase 3 
      activity and percent of cells in sub-G1 phase were increased by B. serrata 
      extract. Cell viability, PGE2 generation, in vitro tube formation and cell 
      migration were decreased significantly in B. serrata-treated HT-29 compared to 
      the control group. CONCLUSION: Our findings suggest that B. serrata extract 
      inhibits proliferation, angiogenesis and migration and induces apoptosis in HT-29 
      cells by inhibiting of mPGES-1 and decreasing the PGE2 level and its downstream 
      targets.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Ranjbarnejad, Tayebeh
AU  - Ranjbarnejad T
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Saidijam, Massoud
AU  - Saidijam M
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Moradkhani, Shirin
AU  - Moradkhani S
AD  - Medicinal Plants and Natural Products Research Center, Hamadan University of 
      Medical Sciences, Hamadan, Iran; Depatment of Pharmacognosy and Pharmaceutical 
      Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Najafi, Rezvan
AU  - Najafi R
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran; Endometrium and Endometriosis Research Center, Hamadan University 
      of Medical Sciences, Hamadan, Iran. Electronic address: re.najafi@umsha.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170524
PL  - United States
TA  - Prostaglandins Other Lipid Mediat
JT  - Prostaglandins & other lipid mediators
JID - 9808648
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Plant Extracts)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 5.3.99.3 (Prostaglandin-E Synthases)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/drug effects
MH  - Boswellia/*chemistry
MH  - Caspase 3/metabolism
MH  - Cell Movement/drug effects
MH  - Colonic Neoplasms/*pathology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - HT29 Cells
MH  - Humans
MH  - Methanol/*chemistry
MH  - Microsomes/drug effects/*enzymology
MH  - *Molecular Targeted Therapy
MH  - Plant Extracts/*pharmacology
MH  - Prostaglandin-E Synthases/*antagonists & inhibitors/biosynthesis
OTO - NOTNLM
OT  - B. serrata extract
OT  - Colorectal cancer
OT  - Microsomal prostaglandin E synthase-1
OT  - Prostaglandin E2
EDAT- 2017/05/28 06:00
MHDA- 2018/05/17 06:00
CRDT- 2017/05/28 06:00
PHST- 2016/10/18 00:00 [received]
PHST- 2017/04/30 00:00 [revised]
PHST- 2017/05/17 00:00 [accepted]
PHST- 2017/05/28 06:00 [pubmed]
PHST- 2018/05/17 06:00 [medline]
PHST- 2017/05/28 06:00 [entrez]
AID - S1098-8823(16)30125-3 [pii]
AID - 10.1016/j.prostaglandins.2017.05.003 [doi]
PST - ppublish
SO  - Prostaglandins Other Lipid Mediat. 2017 Jul;131:1-8. doi: 
      10.1016/j.prostaglandins.2017.05.003. Epub 2017 May 24.