PMID- 28550154
OWN - NLM
STAT- MEDLINE
DCOM- 20180405
LR  - 20181113
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 37
IP  - 4
DP  - 2017 Aug 31
TI  - An amperometric H(2)O(2) biosensor based on hemoglobin nanoparticles immobilized 
      on to a gold electrode.
LID - BSR20170194 [pii]
LID - 10.1042/BSR20170194 [doi]
AB  - The nanoparticles (NPs) of hemoglobin (Hb) were prepared by desolvation method 
      and characterized by transmission electron microscopy (TEM), UV spectroscopy and 
      Fourier-transform IR (FTIR) spectroscopy. An amperometric H(2)O(2) biosensor was 
      constructed by immobilizing HbNPs covalently on to a polycrystalline Au electrode 
      (AuE). HbNPs/AuE were characterized by scanning electron microscopy (SEM), cyclic 
      voltammetry (CV) and electrochemical impedance spectra (EIS) before and after 
      immobilization of HbNPs. The HbNPs/AuE showed optimum response within 2.5 s at pH 
      6.5 in 0.1 M sodium phosphate buffer (PB) containing 100 muM H(2)O(2) at 30 degrees C, 
      when operated at -0.2 V against Ag/AgCl. The HbNPs/AuE exhibited V(max) of 5.161 
      +/- 0.1 muA cm(-2) with apparent Michaelis-Menten constant (K(m)) of 0.1 +/- 0.01 mM. 
      The biosensor showed lower detection limit (1.0 muM), high sensitivity (129 +/- 0.25 
      muA cm(-2) mM(-1)) and wider linear range (1.0-1200 muM) for H(2)O(2) as compared 
      with earlier biosensors. The analytical recoveries of added H(2)O(2) in serum 
      (0.5 and 1.0 muM) were 97.77 and 98.01% respectively, within and between batch 
      coefficients of variation (CV) were 3.16 and 3.36% respectively. There was a good 
      correlation between sera H(2)O(2) values obtained by standard enzymic 
      colorimetric method and the present biosensor (correlation coefficient, R(2) 
      =0.99). The biosensor measured H(2)O(2) level in sera of apparently healthy 
      subjects and persons suffering from diabetes type II. The HbNPs/AuE lost 10% of 
      its initial activity after 90 days of regular use, when stored dry at 4 degrees C.
CI  - (c) 2017 The Author(s).
FAU - Narwal, Vinay
AU  - Narwal V
AD  - Department of Biochemistry, M.D. University, Rohtak, Haryana, India.
FAU - Yadav, Neelam
AU  - Yadav N
AD  - Centre for Biotechnology, M.D. University, Rohtak, Haryana, India.
FAU - Thakur, Manisha
AU  - Thakur M
AD  - Dr B.R. Sur Homeopathic Medical College and Hospital, Department of Pathology, 
      New Delhi, India.
FAU - Pundir, Chandra S
AU  - Pundir CS
AD  - Department of Biochemistry, M.D. University, Rohtak, Haryana, India 
      chandraspundir@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20170716
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Hemoglobins)
RN  - 7440-57-5 (Gold)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Electrodes
MH  - Gold/*chemistry
MH  - Hemoglobins/*chemistry
MH  - Hydrogen Peroxide/*analysis
MH  - Nanoparticles/*chemistry
PMC - PMC5518533
OTO - NOTNLM
OT  - Gold electrode
OT  - H2O2
OT  - H2O2 biosensor
OT  - Hemoglobin nanoparticles
OT  - Serum
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2017/05/28 06:00
MHDA- 2018/04/06 06:00
PMCR- 2017/07/17
CRDT- 2017/05/28 06:00
PHST- 2017/03/02 00:00 [received]
PHST- 2017/05/20 00:00 [revised]
PHST- 2017/05/26 00:00 [accepted]
PHST- 2017/05/28 06:00 [pubmed]
PHST- 2018/04/06 06:00 [medline]
PHST- 2017/05/28 06:00 [entrez]
PHST- 2017/07/17 00:00 [pmc-release]
AID - BSR20170194 [pii]
AID - 10.1042/BSR20170194 [doi]
PST - epublish
SO  - Biosci Rep. 2017 Jul 16;37(4):BSR20170194. doi: 10.1042/BSR20170194. Print 2017 
      Aug 31.