PMID- 28551680
OWN - NLM
STAT- MEDLINE
DCOM- 20170821
LR  - 20191210
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 37
IP  - 6
DP  - 2017 Jun
TI  - RT-PCR for Detecting ALK Translocations in Cytology Samples from Lung Cancer 
      Patients.
PG  - 3295-3299
AB  - BACKGROUND/AIM: We evaluated the usefulness of reverse transcription-polymerase 
      chain reaction (RT-PCR) for detecting anaplastic lymphoma kinase (ALK) 
      translocations using cytology samples from lung cancer patients. MATERIALS AND 
      METHODS: We analyzed ALK translocations by RT-PCR in cytology samples from lung 
      cancer patients diagnosed at the Nippon Medical School Hospital between 2013 and 
      2015. Immunochemistry (IHC) and break-apart fluorescence in situ hybridization 
      (FISH) were also performed on available tissue samples. RESULTS: A total of 155 
      cytology samples were analyzed in our study. We obtained 115 (68%) samples from 
      bronchial lavage. We were able to determine 153 (99%) results by RT-PCR with 4 
      (3%) positive samples. The four samples positive by RT-PCR were also positive by 
      IHC and FISH performed on the tissue samples collected simultaneously. 
      CONCLUSION: RT-PCR is a suitable method for detecting ALK translocations using 
      cytology samples from patients with primary lung cancer, especially when tissue 
      samples are not available.
CI  - Copyright(c) 2017, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Nakamichi, Shinji
AU  - Nakamichi S
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Seike, Masahiro
AU  - Seike M
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan mseike@nms.ac.jp.
FAU - Miyanaga, Akihiko
AU  - Miyanaga A
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Chiba, Mika
AU  - Chiba M
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Matsuda, Kuniko
AU  - Matsuda K
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Kobayashi, Kenichi
AU  - Kobayashi K
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Takahashi, Akiko
AU  - Takahashi A
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Takeuchi, Susumu
AU  - Takeuchi S
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Minegishi, Yuji
AU  - Minegishi Y
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Kubota, Kaoru
AU  - Kubota K
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
FAU - Gemma, Akihiko
AU  - Gemma A
AD  - Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, 
      Nippon Medical School, Tokyo, Japan.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Anaplastic Lymphoma Kinase
MH  - Biomarkers, Tumor/*genetics
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/enzymology/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Predictive Value of Tests
MH  - Receptor Protein-Tyrosine Kinases/*genetics
MH  - Retrospective Studies
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Tokyo
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - ALK
OT  - FISH
OT  - RT-PCR
OT  - cytology samples
OT  - immunochemistry
EDAT- 2017/05/30 06:00
MHDA- 2017/08/22 06:00
CRDT- 2017/05/29 06:00
PHST- 2017/04/13 00:00 [received]
PHST- 2017/04/29 00:00 [revised]
PHST- 2017/05/02 00:00 [accepted]
PHST- 2017/05/29 06:00 [entrez]
PHST- 2017/05/30 06:00 [pubmed]
PHST- 2017/08/22 06:00 [medline]
AID - 37/6/3295 [pii]
AID - 10.21873/anticanres.11696 [doi]
PST - ppublish
SO  - Anticancer Res. 2017 Jun;37(6):3295-3299. doi: 10.21873/anticanres.11696.