PMID- 28551875
OWN - NLM
STAT- MEDLINE
DCOM- 20171023
LR  - 20181113
IS  - 1976-3794 (Electronic)
IS  - 1225-8873 (Linking)
VI  - 55
IP  - 6
DP  - 2017 Jun
TI  - Non-ureolytic calcium carbonate precipitation by Lysinibacillus sp. YS11 isolated 
      from the rhizosphere of Miscanthus sacchariflorus.
PG  - 440-447
LID - 10.1007/s12275-017-7086-z [doi]
AB  - Although microbially induced calcium carbonate precipitation (MICP) through 
      ureolysis has been widely studied in environmental engineering fields, urea 
      utilization might cause environmental problems as a result of ammonia and nitrate 
      production. In this study, many non-ureolytic calcium carbonate-precipitating 
      bacteria that induced an alkaline environment were isolated from the rhizosphere 
      of Miscanthus sacchariflorus near an artificial stream and their ability to 
      precipitate calcium carbonate minerals with the absence of urea was investigated. 
      MICP was observed using a phase-contrast microscope and ion-selective electrode. 
      Only Lysinibacillus sp. YS11 showed MICP in aerobic conditions. Energy dispersive 
      X-ray spectrometry and X-ray diffraction confirmed the presence of calcium 
      carbonate. Field emission scanning electron microscopy analysis indicated the 
      formation of morphologically distinct minerals around cells under these 
      conditions. Monitoring of bacterial growth, pH changes, and Ca(2+) concentrations 
      under aerobic, hypoxia, and anaerobic conditions suggested that strain YS11 could 
      induce alkaline conditions up to a pH of 8.9 and utilize 95% of free Ca(2+) only 
      under aerobic conditions. Unusual Ca(2+) binding and its release from cells were 
      observed under hypoxia conditions. Biofilm and extracellular polymeric substances 
      (EPS) formation were enhanced during MICP. Strain YS11 has resistance at high pH 
      and in high salt concentrations, as well as its spore-forming ability, which 
      supports its potential application for self-healing concrete.
FAU - Lee, Yun Suk
AU  - Lee YS
AD  - Laboratory of Molecular Environmental Microbiology, Department of Environmental 
      Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of 
      Korea.
FAU - Kim, Hyun Jung
AU  - Kim HJ
AD  - Laboratory of Molecular Environmental Microbiology, Department of Environmental 
      Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of 
      Korea.
FAU - Park, Woojun
AU  - Park W
AD  - Laboratory of Molecular Environmental Microbiology, Department of Environmental 
      Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of 
      Korea. wpark@korea.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20170528
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 8W8T17847W (Urea)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacillaceae/growth & development/isolation & purification/*metabolism
MH  - Calcium Carbonate/*metabolism
MH  - Chemical Precipitation
MH  - Microscopy, Electron, Scanning
MH  - Poaceae/*microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rhizosphere
MH  - Spectrometry, X-Ray Emission
MH  - Urea/*metabolism
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - Lysinibacillus sp. YS11
OT  - MICP
OT  - X-ray diffraction
OT  - aeration
OT  - aerobic MICP
OT  - urea
EDAT- 2017/05/30 06:00
MHDA- 2017/10/24 06:00
CRDT- 2017/05/29 06:00
PHST- 2017/02/24 00:00 [received]
PHST- 2017/03/10 00:00 [accepted]
PHST- 2017/03/03 00:00 [revised]
PHST- 2017/05/29 06:00 [entrez]
PHST- 2017/05/30 06:00 [pubmed]
PHST- 2017/10/24 06:00 [medline]
AID - 10.1007/s12275-017-7086-z [pii]
AID - 10.1007/s12275-017-7086-z [doi]
PST - ppublish
SO  - J Microbiol. 2017 Jun;55(6):440-447. doi: 10.1007/s12275-017-7086-z. Epub 2017 
      May 28.