PMID- 2855290
OWN - NLM
STAT- MEDLINE
DCOM- 19890801
LR  - 20190828
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 19
IP  - 3-4
DP  - 1988 Oct
TI  - Molecular studies of T-lymphocytes from cattle infected with bovine leukemia 
      virus.
PG  - 307-23
AB  - Although bovine leukemia virus (BLV) is mainly associated with infections of 
      B-lymphocytes, we have previously reported the statistically significant increase 
      in the T-lymphocytes obtained from BLV-infected asymptomatic aleukemic (AL) 
      cattle. In this report the presence of BLV provirus in the DNA of immunoaffinity 
      purified T-lymphocytes from AL animals was assessed using a highly specific 
      radiolabelled (32P) BLV-DNA provirus probe and solid phase DNA hybridization. The 
      BLV provirus was found in the DNA of the peripheral blood mononuclear cells of 
      all AL animals tested and three of the four purified T-lymphocyte preparations 
      from these animals. The purified T-lymphocyte preparations used in this study 
      contained less than 4% detectable B-lymphocytes. One animal had no detectable 
      B-lymphocytes in the purified T-lymphocyte preparation and the DNA from these 
      cells also gave positive hybridization results. The lymphocyte blastogenesis 
      assay was then used as an indicator of the functional ability of lymphocytes from 
      these BLV-infected AL cattle to respond to mitogenic stimuli. The responsiveness 
      of lymphocytes from these animals to the mitogens concanavalin A (Con A), 
      phytohemagglutinin (PHA), and pokeweek mitogen (PWM) was comparable to that of 
      lymphocytes from BLV-negative animals when changes in 3H-thymidine uptake 
      (c.p.m.) were used as measurement of mitogenic-induced blastogenesis. This 
      indicated that infection of the T-lymphocytes by BLV does not appear to alter the 
      overall response of the lymphocyte populations to mitogenic stimuli. High levels 
      of spontaneous blastogenesis in the absence of mitogenic stimulation were 
      observed for lymphocyte preparations of AL animals. The reason for this 
      proliferation of lymphocytes is unclear; however, sera from these AL animals were 
      found to contain a blastogenesis-augmenting factor(s) when added to lymphocytes 
      from BLV-negative control animals in the presence of Con A, PHA and PWM.
FAU - Williams, D L
AU  - Williams DL
AD  - Department of Veterinary Microbiology and Parasitology, School of Veterinary 
      Medicine, Louisiana State University, Baton Rouge 70803.
FAU - Barta, O
AU  - Barta O
FAU - Amborski, G F
AU  - Amborski GF
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*immunology/microbiology
MH  - DNA, Viral/isolation & purification
MH  - Female
MH  - Leukemia/immunology/microbiology/*veterinary
MH  - Leukemia Virus, Bovine/isolation & purification
MH  - Lymphocyte Activation
MH  - Proviruses/isolation & purification
MH  - T-Lymphocytes/*immunology/microbiology
EDAT- 1988/10/01 00:00
MHDA- 1988/10/01 00:01
CRDT- 1988/10/01 00:00
PHST- 1988/10/01 00:00 [pubmed]
PHST- 1988/10/01 00:01 [medline]
PHST- 1988/10/01 00:00 [entrez]
AID - 0165-2427(88)90117-1 [pii]
AID - 10.1016/0165-2427(88)90117-1 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1988 Oct;19(3-4):307-23. doi: 
      10.1016/0165-2427(88)90117-1.