PMID- 28553277
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Structural Analysis of Porcine Reproductive and Respiratory Syndrome Virus 
      Non-structural Protein 7alpha (NSP7alpha) and Identification of Its Interaction with 
      NSP9.
PG  - 853
LID - 10.3389/fmicb.2017.00853 [doi]
LID - 853
AB  - Non-structural protein 7 (NSP7), which can be further cleaved into NSP7alpha and 
      NSP7beta, is one of the most conserved proteins of porcine reproductive and 
      respiratory syndrome virus (PRRSV). NSP7 plays a role in provoking the humoral 
      immune system in PRRSV-infected swine, but its structure and function are still 
      not fully understood. Here, we analyzed the expression of NSP7, NSP7alpha, and NSP7beta 
      in PRRSV-infected MARC-145 cells. The solution structure of NSP7alpha was determined 
      by using nuclear magnetic resonance (NMR). Although the structure provided little 
      clue to its function, based on the structure of NSP7alpha, we predicted and further 
      identified some key amino acids on NSP7alpha for the interaction of NSP7alpha with NSP9, 
      the RNA dependent RNA polymerase of PRRSV. This study provided some new insights 
      into the structure and function of PRRSV NSP7.
FAU - Chen, Jiaping
AU  - Chen J
AD  - College of Life Sciences, Northwest A&F UniversityYangling, China.
FAU - Xu, Xiaodong
AU  - Xu X
AD  - College of Life Sciences, Northwest A&F UniversityYangling, China.
FAU - Tao, Hu
AU  - Tao H
AD  - College of Science, Northwest A&F UniversityYangling, China.
FAU - Li, Yuan
AU  - Li Y
AD  - College of Life Sciences, Northwest A&F UniversityYangling, China.
FAU - Nan, Hao
AU  - Nan H
AD  - College of Life Sciences, Northwest A&F UniversityYangling, China.
FAU - Wang, Yuanyuan
AU  - Wang Y
AD  - College of Life Sciences, Northwest A&F UniversityYangling, China.
FAU - Tian, Mengmeng
AU  - Tian M
AD  - College of Life Sciences, Northwest A&F UniversityYangling, China.
FAU - Chen, Hongying
AU  - Chen H
AD  - College of Life Sciences, Northwest A&F UniversityYangling, China.
LA  - eng
PT  - Journal Article
DEP - 20170511
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5425468
OTO - NOTNLM
OT  - NMR
OT  - non-structural protein 7alpha
OT  - porcine reproductive and respiratory syndrome virus
OT  - protein structure
OT  - protein-protein interaction
EDAT- 2017/05/30 06:00
MHDA- 2017/05/30 06:01
PMCR- 2017/05/11
CRDT- 2017/05/30 06:00
PHST- 2017/03/12 00:00 [received]
PHST- 2017/04/26 00:00 [accepted]
PHST- 2017/05/30 06:00 [entrez]
PHST- 2017/05/30 06:00 [pubmed]
PHST- 2017/05/30 06:01 [medline]
PHST- 2017/05/11 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.00853 [doi]
PST - epublish
SO  - Front Microbiol. 2017 May 11;8:853. doi: 10.3389/fmicb.2017.00853. eCollection 
      2017.