PMID- 28554614
OWN - NLM
STAT- MEDLINE
DCOM- 20170810
LR  - 20181202
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 235
DP  - 2017 Nov 15
TI  - Antibiotic use in heavy pigs: Comparison between urine and muscle samples from 
      food chain animals analysed by HPLC-MS/MS.
PG  - 111-118
LID - S0308-8146(17)30769-0 [pii]
LID - 10.1016/j.foodchem.2017.04.184 [doi]
AB  - The antibiotic overuse in zoothechnics, due to prophylactic and therapeutic 
      treatments, or to their growth-promoting activity, is a major cause for the onset 
      of widespread antibiotic resistance. Of particular relevance to this study, is 
      the antibiotic abuse in pig breeding. Despite the comprehensive literature on 
      residue controls in pig muscle, data on pig urine, a non-invasive, on-farm 
      collectable matrix, are lacking. Therefore, we validated an HPLC-MS/MS method to 
      detect 29 antimicrobials from eight classes and applied it to 43 anonymous pig 
      urine and muscle paired samples and fulfilled the parameters in agreement with 
      the Commission Decision 2002/657/UE. The analytical limits were moreover much 
      lower than the maximum residue limits (MRLs) required by the Commission 
      Regulation 37/2010/UE. In the samples, antibiotics were usually detected at 
      higher frequencies and concentrations in urine than muscle. Urine proved a useful 
      tool to detect antibiotic administration and their excessive use in pig farming 
      is depicted.
CI  - Copyright (c) 2017. Published by Elsevier Ltd.
FAU - Chiesa, Luca Maria
AU  - Chiesa LM
AD  - Department of Health, Animal Science and Food Safety, University of Milan, Via 
      Celoria 10, 20133 Milan, Italy.
FAU - Nobile, Maria
AU  - Nobile M
AD  - Department of Health, Animal Science and Food Safety, University of Milan, Via 
      Celoria 10, 20133 Milan, Italy.
FAU - Panseri, Sara
AU  - Panseri S
AD  - Department of Health, Animal Science and Food Safety, University of Milan, Via 
      Celoria 10, 20133 Milan, Italy. Electronic address: sara.panseri@unimi.it.
FAU - Arioli, Francesco
AU  - Arioli F
AD  - Department of Health, Animal Science and Food Safety, University of Milan, Via 
      Celoria 10, 20133 Milan, Italy.
LA  - eng
PT  - Journal Article
DEP - 20170502
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*administration & dosage/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Drug Resistance, Bacterial
MH  - Food Chain
MH  - Muscle, Skeletal/*chemistry
MH  - Swine/*metabolism
MH  - Tandem Mass Spectrometry
MH  - Urinalysis/*veterinary
OTO - NOTNLM
OT  - Amoxicillin (PubChemCID: 33613)
OT  - Ampicillin (PubChemCID: 6249)
OT  - Antibiotics
OT  - Benzylpenicillin (PubChemCID: 5904)
OT  - Cefalexin (PubChemCID: 27447)
OT  - Cefquinome sulphate (PubChemCID: 9577261)
OT  - Chloramphenicol (PubChemCID: 5959)
OT  - Chlortetracycline (PubChemCID: 54737570)
OT  - Ciprofloxacin (PubChemCID: 2764)
OT  - Cloxacillin (PubChemCID: 6098)
OT  - Dicloxacillin (PubChemCID: 18381)
OT  - Doxycycline hyclate (PubChemCID: 54686183)
OT  - Enrofloxacin (PubChemCID: 71188)
OT  - Erythromycin (PubChemCID: 12560)
OT  - Florfenicol (PubChemCID: 114811)
OT  - Florfenicol amine (PubChemCID: 156406)
OT  - Flumequine (PubChemCID: 3374)
OT  - HPLC-MS/MS
OT  - Lincomycin (PubChemCID: 3000540)
OT  - Lomefloxacin hydrochloride (PubChemCID: 68624)
OT  - Marbofloxacin (PubChemCID: 60651)
OT  - Muscle tissue
OT  - Nalidixic acid (PubChemCID: 4421)
OT  - Oxolinic acid (PubChemCID: 4628)
OT  - Oxytetracycline (PubChemCID: 54675779)
OT  - Sulfadiazine (PubChemCID: 441244)
OT  - Sulfadimethoxine (PubChemCID: 5323)
OT  - Sulfadimidine (PubChemCID: 5327)
OT  - Sulfathiazole (PubChemCID: 5340)
OT  - Swine
OT  - Tetracycline hydrochloride (PubChemCID: 54704426)
OT  - Trimethoprim (PubChemCID: 5578)
OT  - Tylosin (PubChemCID: 5280440).
OT  - Urine
EDAT- 2017/05/31 06:00
MHDA- 2017/08/11 06:00
CRDT- 2017/05/31 06:00
PHST- 2016/12/06 00:00 [received]
PHST- 2017/02/23 00:00 [revised]
PHST- 2017/04/29 00:00 [accepted]
PHST- 2017/05/31 06:00 [entrez]
PHST- 2017/05/31 06:00 [pubmed]
PHST- 2017/08/11 06:00 [medline]
AID - S0308-8146(17)30769-0 [pii]
AID - 10.1016/j.foodchem.2017.04.184 [doi]
PST - ppublish
SO  - Food Chem. 2017 Nov 15;235:111-118. doi: 10.1016/j.foodchem.2017.04.184. Epub 
      2017 May 2.