PMID- 28554841
OWN - NLM
STAT- MEDLINE
DCOM- 20170925
LR  - 20181202
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 489
IP  - 3
DP  - 2017 Jul 29
TI  - ERK1/2 signaling mediated naringin-induced osteogenic differentiation of 
      immortalized human periodontal ligament stem cells.
PG  - 319-325
LID - S0006-291X(17)31023-9 [pii]
LID - 10.1016/j.bbrc.2017.05.130 [doi]
AB  - Periodontal ligament stem cells (PDLSCs) are promising tools for the 
      investigations of cell differentiation and bone regeneration. However, the 
      limited life span significantly restricts their usefulness. In this study, we 
      established an immortalized PDLSC cell line by the introduction of Bmi1 
      (PDLSC-Bmi1). Several genes related to cell cycle, cell replication and stemness 
      were found to be changed with the overexpression of Bmi1. Compared with primary 
      PDLSCs, the immortalized cells had a slower aging rate, maintained in a 
      proliferative state without crisis for more than 30 passages, and retained the 
      molecular markers and biological functions of primary ones. Using the PDLSC-Bmi1, 
      we confirmed the promotive effect of naringin on osteogenesis. Naringin promoted 
      the osteogenic differentiation of PDLSC-Bmi1 manifested as the increased activity 
      of alkaline phosphatase (ALP), expression of the runt-related transcription 
      factor 2 (Runx2) and osteocalcin (OCN), and formation of mineralized nodules. In 
      addition, the extracellular regulated protein kinases (ERK) 1/2 was found to be 
      activated by naringin, and the ERK1/2 specific inhibitor significantly inhibited 
      naringin-induced osteogenic differentiation in PDLSC-Bmi1. Our results indicated 
      that the overexpression of Bmi1 extended the life span of PDLSCs without 
      perturbing their biological functions, and that naringin promoted the 
      osteogenesis of PDLSC-Bmi1 at least partially through the ERK1/2 signaling 
      pathway.
CI  - Copyright (c) 2017 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Wei, Kai
AU  - Wei K
AD  - Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of 
      Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research 
      Center for Kidney Diseases, Beijing 100853, China; Medical College, Nankai 
      University, Tianjin 300071, China.
FAU - Xie, Yuansheng
AU  - Xie Y
AD  - Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of 
      Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research 
      Center for Kidney Diseases, Beijing 100853, China. Electronic address: 
      xieyuansn@hotmail.com.
FAU - Chen, Tianyu
AU  - Chen T
AD  - Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key 
      Laboratory of Stomatology, Guangzhou 510080, China.
FAU - Fu, Bo
AU  - Fu B
AD  - Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of 
      Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research 
      Center for Kidney Diseases, Beijing 100853, China.
FAU - Cui, Shaoyuan
AU  - Cui S
AD  - Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of 
      Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research 
      Center for Kidney Diseases, Beijing 100853, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key 
      Laboratory of Stomatology, Guangzhou 510080, China.
FAU - Cai, Guangyan
AU  - Cai G
AD  - Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of 
      Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research 
      Center for Kidney Diseases, Beijing 100853, China.
FAU - Chen, Xiangmei
AU  - Chen X
AD  - Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of 
      Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research 
      Center for Kidney Diseases, Beijing 100853, China.
LA  - eng
PT  - Journal Article
DEP - 20170526
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Flavanones)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - N7TD9J649B (naringin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cell Differentiation/drug effects
MH  - Female
MH  - Flavanones/chemistry/*pharmacology
MH  - HEK293 Cells
MH  - Humans
MH  - MAP Kinase Signaling System/*drug effects
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/*drug effects
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Osteogenesis/*drug effects
MH  - Periodontal Ligament/*cytology
MH  - Young Adult
OTO - NOTNLM
OT  - ERK
OT  - Immortalization
OT  - Naringin
OT  - Osteogenesis
OT  - Periodontal ligament stem cell
EDAT- 2017/05/31 06:00
MHDA- 2017/09/26 06:00
CRDT- 2017/05/31 06:00
PHST- 2017/03/17 00:00 [received]
PHST- 2017/05/23 00:00 [accepted]
PHST- 2017/05/31 06:00 [pubmed]
PHST- 2017/09/26 06:00 [medline]
PHST- 2017/05/31 06:00 [entrez]
AID - S0006-291X(17)31023-9 [pii]
AID - 10.1016/j.bbrc.2017.05.130 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Jul 29;489(3):319-325. doi: 
      10.1016/j.bbrc.2017.05.130. Epub 2017 May 26.