PMID- 28557150
OWN - NLM
STAT- MEDLINE
DCOM- 20190307
LR  - 20190307
IS  - 1524-4741 (Electronic)
IS  - 1075-122X (Linking)
VI  - 24
IP  - 1
DP  - 2018 Jan
TI  - Characterization of CD34-deficient myofibroblastomas of the breast.
PG  - 55-61
LID - 10.1111/tbj.12835 [doi]
AB  - Mammary myofibroblastoma is a benign spindle cell tumor that can show variable 
      morphologic patterns and lines of differentiation. Diffuse and strong 
      immunohistochemical expression of CD34 is a characteristic of myofibroblastoma 
      and greatly aids in confirming a diagnosis. Myofibroblastoma has been shown to 
      belong to a family of CD34-positive tumors with similar morphology that show a 
      deletion of 13q14, which includes RB1 and FOXO1A genes. The purpose of this study 
      was to better understand a subset of myofibroblastomas that is characteristically 
      CD34-deficient by immunohistochemistry. Six myofibroblastomas were studied by 
      immunohistochemistry and fluorescence in situ hybridization (FISH) for RB1. 
      Patients included five women and one man, aged 41-85 years (median, 52.5). Tumor 
      size ranged from 0.4 to 1.5 cm (mean, 0.95). Tumors showed spindle cell 
      morphology in five cases and epithelioid features in one case. Two tumors showed 
      complete lack of CD34 staining. The remaining showed weak focal or weak patchy 
      CD34 staining. Dichotomous staining was seen in one case with CD34-positive 
      spindle cell areas and CD34-negative myxoid areas. All six tumors showed ER 
      expression, five of six showed desmin expression, and four of six showed bcl-2 
      positivity. Two of six (33.3%) tumors showed deletion of RB1 by FISH, including 
      one that showed loss of Rb immunohistochemical staining. Myofibroblastomas 
      uncommonly show absent/focal expression of CD34, a potential diagnostic pitfall, 
      particularly in small samples. Characteristic staining with other 
      immunohistochemical markers is seen which can aid in confirming the diagnosis. 
      These tumors may harbor deletion of RB1, similar to CD34-positive 
      myofibroblastomas, and this deletion may not correlate with loss of Rb by 
      immunohistochemistry.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - D'Alfonso, Timothy M
AU  - D'Alfonso TM
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New 
      York, NY, USA.
FAU - Subramaniyam, Shivakumar
AU  - Subramaniyam S
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New 
      York, NY, USA.
FAU - Ginter, Paula S
AU  - Ginter PS
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New 
      York, NY, USA.
FAU - Mosquera, Juan Miguel
AU  - Mosquera JM
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New 
      York, NY, USA.
AD  - Institute for Precision Medicine of Weill Cornell and New York-Presbyterian 
      Hospital, New York, NY, USA.
FAU - Croyle, Jaclyn
AU  - Croyle J
AD  - Institute for Precision Medicine of Weill Cornell and New York-Presbyterian 
      Hospital, New York, NY, USA.
FAU - Liu, Yi-Fang
AU  - Liu YF
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New 
      York, NY, USA.
FAU - Rubin, Mark A
AU  - Rubin MA
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New 
      York, NY, USA.
AD  - Institute for Precision Medicine of Weill Cornell and New York-Presbyterian 
      Hospital, New York, NY, USA.
FAU - Shin, Sandra J
AU  - Shin SJ
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New 
      York, NY, USA.
LA  - eng
PT  - Journal Article
DEP - 20170530
PL  - United States
TA  - Breast J
JT  - The breast journal
JID - 9505539
RN  - 0 (Antigens, CD34)
RN  - 0 (Biomarkers, Tumor)
RN  - 13q deletion syndrome
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD34/*metabolism
MH  - Biomarkers, Tumor/metabolism
MH  - Breast Neoplasms/genetics/*pathology
MH  - Breast Neoplasms, Male/genetics/pathology
MH  - Chromosome Deletion
MH  - Chromosome Disorders/complications/genetics
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasms, Muscle Tissue/genetics/*pathology
OTO - NOTNLM
OT  - 13q14
OT  - CD34
OT  - RB1
OT  - myofibroblastoma
EDAT- 2017/05/31 06:00
MHDA- 2019/03/08 06:00
CRDT- 2017/05/31 06:00
PHST- 2016/07/19 00:00 [received]
PHST- 2016/10/18 00:00 [revised]
PHST- 2016/10/18 00:00 [accepted]
PHST- 2017/05/31 06:00 [pubmed]
PHST- 2019/03/08 06:00 [medline]
PHST- 2017/05/31 06:00 [entrez]
AID - 10.1111/tbj.12835 [doi]
PST - ppublish
SO  - Breast J. 2018 Jan;24(1):55-61. doi: 10.1111/tbj.12835. Epub 2017 May 30.