PMID- 28557173 OWN - NLM STAT- MEDLINE DCOM- 20171229 LR - 20180122 IS - 1439-7633 (Electronic) IS - 1439-4227 (Linking) VI - 18 IP - 16 DP - 2017 Aug 17 TI - A Fluorescent RNA Forced-Intercalation Probe as a Pan-Selective Marker for Influenza A Virus Infection. PG - 1589-1592 LID - 10.1002/cbic.201700271 [doi] AB - The influenza A virus (IAV) genome is segmented into eight viral ribonucleoproteins, each expressing a negatively oriented viral RNA (vRNA). Along the infection cycle, highly abundant single-stranded small viral RNAs (svRNA) are transcribed in a segment-specific manner. The sequences of svRNAs and of the vRNA 5'-ends are identical and highly conserved among all IAV strains. Here, we demonstrate that these sequences can be used as a target for a pan-selective sensor of IAV infection. To this end, we used a complementary fluorescent forced-intercalation RNA (IAV QB-FIT) probe with a single locked nucleic acid substitution to increase brightness. We demonstrated by fluorescence in situ hybridization (FISH) that this probe is suitable and easy to use to detect infection of different cell types by a broad variety of avian, porcine, and human IAV strains, but not by other influenza virus types. IAV QB-FIT also provides a useful tool to characterize different infection states of the host cell. CI - (c) 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. FAU - Haralampiev, Ivan AU - Haralampiev I AD - Institut fur Biologie, Molekulare Biophysik, IRI Life Sciences, Humboldt-Universitat zu Berlin, Invalidenstrasse 42, 10115, Berlin, Germany. FAU - Schade, Matthias AU - Schade M AD - Institut fur Biologie, Molekulare Biophysik, IRI Life Sciences, Humboldt-Universitat zu Berlin, Invalidenstrasse 42, 10115, Berlin, Germany. FAU - Chamiolo, Jasmine AU - Chamiolo J AD - Institut fur Chemie, Bioorganische Synthese, Humboldt-Universitat zu Berlin, Brook-Taylor-Strasse 2, 12489, Berlin, Germany. FAU - Jolmes, Fabian AU - Jolmes F AD - Institut fur Biologie, Molekulare Biophysik, IRI Life Sciences, Humboldt-Universitat zu Berlin, Invalidenstrasse 42, 10115, Berlin, Germany. FAU - Prisner, Simon AU - Prisner S AUID- ORCID: 0000-0003-0559-2271 AD - Institut fur Biologie, Molekulare Biophysik, IRI Life Sciences, Humboldt-Universitat zu Berlin, Invalidenstrasse 42, 10115, Berlin, Germany. FAU - Witkowski, Peter T AU - Witkowski PT AD -