PMID- 28557303
OWN - NLM
STAT- MEDLINE
DCOM- 20181112
LR  - 20210109
IS  - 1751-7915 (Electronic)
IS  - 1751-7915 (Linking)
VI  - 11
IP  - 1
DP  - 2018 Jan
TI  - Simultaneous fermentation of cellulose and current production with an enriched 
      mixed culture of thermophilic bacteria in a microbial electrolysis cell.
PG  - 63-73
LID - 10.1111/1751-7915.12733 [doi]
AB  - An enriched mixed culture of thermophilic (60 degrees C) bacteria was assembled for the 
      purpose of using cellulose to produce current in thermophilic microbial 
      electrolysis cells (MECs). Cellulose was fermented into sugars and acids before 
      being consumed by anode-respiring bacteria (ARB) for current production. Current 
      densities (j) were sustained at 6.5 +/- 0.2 A m(-2) in duplicate reactors with a 
      coulombic efficiency (CE) of 84 +/- 0.3%, a coulombic recovery (CR) of 54 +/- 11% and 
      without production of CH(4) . Low-scan rate cyclic voltammetry (LSCV) revealed a 
      mid-point potential (E(ka) ) of -0.17 V versus SHE. Pyrosequencing analysis of 
      the V4 hypervariable region of 16S rDNA and scanning electron microscopy present 
      an enriched thermophilic microbial community consisting mainly of the phylum 
      Firmicutes with the Thermoanaerobacter (46 +/- 13%) and Thermincola (28 +/- 14%) 
      genera occupying the biofilm anode in high relative abundance and Tepidmicrobium 
      (38 +/- 6%) and Moorella (11 +/- 8%) genera present in high relative abundance in the 
      bulk medium. The Thermoanaerobacter (15 +/- 16%) and Brevibacillus (21 +/- 30%) 
      genera were also present in the bulk medium; however, their relative abundance 
      varied by reactor. This study indicates that thermophilic consortia can obtain 
      high CE and CR, while sustaining high current densities from cellulose in MECs.
CI  - (c) 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd 
      and Society for Applied Microbiology.
FAU - Lusk, Bradley G
AU  - Lusk BG
AD  - Biodesign Swette Center for Environmental Biotechnology, Arizona State 
      University, P.O. Box 875701, Tempe, AZ, 85287-5701, USA.
AD  - #ScienceTheEarth, Mesa, AZ, 85201, USA.
FAU - Colin, Alexandra
AU  - Colin A
AD  - Ecole Normale Superieure, 45, rue d'Ulm, 75230, Paris Cedex 05, France.
FAU - Parameswaran, Prathap
AU  - Parameswaran P
AD  - Department of Civil Engineering, Kansas State University, 2123 Fiedler Hall, 
      Manhattan, KS, 66502, USA.
FAU - Rittmann, Bruce E
AU  - Rittmann BE
AD  - Biodesign Swette Center for Environmental Biotechnology, Arizona State 
      University, P.O. Box 875701, Tempe, AZ, 85287-5701, USA.
AD  - School of Sustainable Engineering and the Built Environment, Arizona State 
      University, Tempe, AZ, USA.
FAU - Torres, Cesar I
AU  - Torres CI
AD  - Biodesign Swette Center for Environmental Biotechnology, Arizona State 
      University, P.O. Box 875701, Tempe, AZ, 85287-5701, USA.
AD  - School for Engineering of Matter, Transport and Energy, Arizona State University, 
      Tempe, AZ, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170529
PL  - United States
TA  - Microb Biotechnol
JT  - Microbial biotechnology
JID - 101316335
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Bacteria/classification/genetics/*metabolism
MH  - *Bioelectric Energy Sources
MH  - Bioreactors/microbiology
MH  - Cellulose/*metabolism
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - *Electricity
MH  - Fermentation
MH  - *Microbial Consortia
MH  - Microscopy, Electron, Scanning
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
PMC - PMC5743814
EDAT- 2017/05/31 06:00
MHDA- 2018/11/13 06:00
PMCR- 2017/05/29
CRDT- 2017/05/31 06:00
PHST- 2016/11/11 00:00 [received]
PHST- 2017/04/26 00:00 [revised]
PHST- 2017/04/27 00:00 [accepted]
PHST- 2017/05/31 06:00 [pubmed]
PHST- 2018/11/13 06:00 [medline]
PHST- 2017/05/31 06:00 [entrez]
PHST- 2017/05/29 00:00 [pmc-release]
AID - MBT212733 [pii]
AID - 10.1111/1751-7915.12733 [doi]
PST - ppublish
SO  - Microb Biotechnol. 2018 Jan;11(1):63-73. doi: 10.1111/1751-7915.12733. Epub 2017 
      May 29.