PMID- 28557870
OWN - NLM
STAT- MEDLINE
DCOM- 20190918
LR  - 20190918
IS  - 1559-0488 (Electronic)
IS  - 1559-047X (Linking)
VI  - 39
IP  - 1
DP  - 2018 Jan 1
TI  - In Situ Delivery of Fibrin-Based Hydrogels Prevents Contraction and Reduces 
      Inflammation.
PG  - 40-53
LID - 10.1097/BCR.0000000000000576 [doi]
AB  - While early excision and grafting has revolutionized burn wound care, autologous 
      split-thickness skin grafts are sometimes unavailable. Tissue-engineered skin 
      substitutes have generated great interest but have proven inadequate. Therefore, 
      the development of novel biomaterials to replace/augment skin grafting could 
      improve burn patient outcomes. Herein, we establish the effects of debridement on 
      deep-partial thickness burns and subsequently examine the effects of 3 different 
      hydrogels on healing. Burns were created on the dorsum of pigs and 4 days after, 
      the eschar was either left intact or debrided for treatment with collagen, 
      PEGylated fibrinogen (PEG-fibrin) or PEGylated autologous platelet-free plasma 
      (PEG-PFP) hydrogels. Wounds were photographed, scored, and biopsied for histology 
      on postburn days 7, 10, 14, and 28. Compared with nondebrided wounds, debridement 
      improved wound color and suppleness but accelerated contraction. Debridement also 
      significantly reduced the number of neutrophils in the wound bed at days 10 and 
      14 postburn. Treatment with any hydrogel transiently mitigated contraction, with 
      the PEG-fibrin group displaying less contraction on day 28. All hydrogels were 
      visible histologically for up to 10 days, with significant cellular and blood 
      vessel infiltration observed in PEG-fibrin hydrogels. Collagen and PEG-fibrin 
      hydrogels reduced neutrophils and macrophages in surrounding granulation tissue 
      on day 7, while PEG-fibrin hydrogels contained less immune cells. These data 
      suggest that a single hydrogel application at the time of debridement has 
      immunomodulatory properties that aid in wound healing. Ultimately, these 
      hydrogels may be combined with other biomaterials, cells, or biologics for 
      replacing/augmenting skin substitutes.
FAU - Burmeister, David M
AU  - Burmeister DM
AD  - Burn Injury Research Task Area, United States Army Institute of Surgical 
      Research, Fort Sam Houston, TX.
FAU - Roy, Daniel C
AU  - Roy DC
AD  - Burn Injury Research Task Area, United States Army Institute of Surgical 
      Research, Fort Sam Houston, TX.
FAU - Becerra, Sandra C
AU  - Becerra SC
AD  - Burn Injury Research Task Area, United States Army Institute of Surgical 
      Research, Fort Sam Houston, TX.
FAU - Natesan, Shanmugasundaram
AU  - Natesan S
AD  - Burn Injury Research Task Area, United States Army Institute of Surgical 
      Research, Fort Sam Houston, TX.
FAU - Christy, Robert J
AU  - Christy RJ
AD  - Burn Injury Research Task Area, United States Army Institute of Surgical 
      Research, Fort Sam Houston, TX.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Burn Care Res
JT  - Journal of burn care & research : official publication of the American Burn 
      Association
JID - 101262774
RN  - 0 (Hydrogels)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9001-31-4 (Fibrin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Burns/*pathology/*therapy
MH  - Collagen
MH  - Debridement
MH  - Disease Models, Animal
MH  - Female
MH  - Fibrin/*therapeutic use
MH  - Hydrogels/*therapeutic use
MH  - Plasma
MH  - Polyethylene Glycols
MH  - Swine
MH  - *Wound Healing
EDAT- 2017/05/31 06:00
MHDA- 2019/09/19 06:00
CRDT- 2017/05/31 06:00
PHST- 2018/01/31 00:00 [received]
PHST- 2018/04/11 00:00 [accepted]
PHST- 2017/05/31 06:00 [pubmed]
PHST- 2019/09/19 06:00 [medline]
PHST- 2017/05/31 06:00 [entrez]
AID - 4775265 [pii]
AID - 10.1097/BCR.0000000000000576 [doi]
PST - ppublish
SO  - J Burn Care Res. 2018 Jan 1;39(1):40-53. doi: 10.1097/BCR.0000000000000576.