PMID- 28558380
OWN - NLM
STAT- MEDLINE
DCOM- 20170711
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 42
IP  - 1
DP  - 2017
TI  - Circulating Tumor Cells as a Biomarker in Pancreatic Ductal Adenocarcinoma.
PG  - 373-382
LID - 10.1159/000477481 [doi]
AB  - BACKGROUND/AIMS: Circulating tumor cells (CTCs) are valuable in both basic 
      research and clinical application for cancer management. In the current study, we 
      evaluated the diagnostic value of CTCs in pancreatic ductal adenocarcinoma 
      (PDAC). METHODS: In total, 143 blood samples from 95 consecutively diagnosed PDAC 
      patients and 48 healthy donors were collected. Combined data from immunostaining 
      of CD45, DAPI and fluorescence in situ hybridization (FISH) with chromosome 8 
      centromere (CEP8) probe were used to identify CTCs. Cells with features of 
      CD45-/DAPI+/CEP8>2 were detected as CTCs. RESULTS: CTCs were classified as 
      triploid, tetraploid and multiploid based on chromosome 8 copy number. CTC 
      subtype composition was significantly different among groups. Both subtype number 
      and total CTC number were significantly increased in PDAC patients, compared to 
      healthy controls. Total CTC number had 75.8% sensitivity and 68.7% specificity at 
      a cutoff value of 2 cells/3.2 mL. This study is the first to report that CTC 
      subtype number is also useful in cancer diagnosis. Sensitivity was 53.7% and 
      specificity was 85.4% at a cutoff point of 2 CTC subtypes. The diagnostic value 
      of both total CTC number and CTC subtype number was a little poorer than CA199. 
      CONCLUSIONS: Both CTC subtype and total CTC number may serve as potential 
      biomarkers for PDAC.
CI  - (c) 2017 The Author(s). Published by S. Karger AG, Basel.
FAU - Liu, Han
AU  - Liu H
AD  - Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, 
      China.
FAU - Sun, Bo
AU  - Sun B
AD  - Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, 
      China.
FAU - Wang, Shengnan
AU  - Wang S
AD  - Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, 
      China.
FAU - Liu, Congjin
AU  - Liu C
AD  - Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, 
      China.
FAU - Lu, Yun
AU  - Lu Y
AD  - Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, 
      China.
FAU - Li, Ding
AU  - Li D
AD  - Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, 
      China.
FAU - Liu, Xingdang
AU  - Liu X
AD  - Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20170525
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Antigens, Tumor-Associated, Carbohydrate)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (carbohydrate antigen 199, human)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Aged
MH  - Antigens, Tumor-Associated, Carbohydrate/blood
MH  - Area Under Curve
MH  - Biomarkers, Tumor/*metabolism
MH  - Carcinoma, Pancreatic Ductal/*diagnosis/metabolism/pathology
MH  - Case-Control Studies
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocyte Common Antigens/metabolism
MH  - Luminescent Measurements
MH  - Male
MH  - Middle Aged
MH  - Neoplastic Cells, Circulating/*metabolism
MH  - Pancreatic Neoplasms/*diagnosis/metabolism/pathology
MH  - ROC Curve
MH  - Tetraploidy
MH  - Triploidy
OTO - NOTNLM
OT  - Aneuploidy
OT  - Biomarker
OT  - Circulating tumor cells
OT  - Pancreatic cancer
EDAT- 2017/05/31 06:00
MHDA- 2017/07/14 06:00
CRDT- 2017/05/31 06:00
PHST- 2016/09/26 00:00 [received]
PHST- 2017/03/23 00:00 [accepted]
PHST- 2017/05/31 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2017/05/31 06:00 [entrez]
AID - 000477481 [pii]
AID - 10.1159/000477481 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2017;42(1):373-382. doi: 10.1159/000477481. Epub 2017 May 
      25.