PMID- 28559136 OWN - NLM STAT- MEDLINE DCOM- 20170925 LR - 20171130 IS - 1090-2104 (Electronic) IS - 0006-291X (Linking) VI - 489 IP - 3 DP - 2017 Jul 29 TI - Intradiencephalon injection of histamine inhibited the recovery of locomotor function of spinal cord injured zebrafish. PG - 275-280 LID - S0006-291X(17)31067-7 [pii] LID - 10.1016/j.bbrc.2017.05.158 [doi] AB - Human spinal cord injury (SCI) usually causes irreversible disability beneath the injured site due to poor neural regeneration. On the contrary, zebrafish show significant regenerative ability after SCI, thus is usually worked as an animal model for studying neuroregeneration. Most of the previous SCI studies focused on the local site of SCI, the supraspinal-derived signals were rarely mentioned. Here we showed that intradiencephalon injection of histamine (HA) inhibited the locomotor recovery in adult zebrafish post-SCI. Immunofluorescence results showed that intradiencephalon HA administration increased the activated microglia 3 days post injury (dpi), promoted the proliferation of radial glial cells at 7 dpi and affected the morphology of radial glial cells at 11 dpi. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) results showed that intradiencephalon HA administration also reduced the expression of neurotrophic factors including brain-derived neurotrophic factor (BDNF) and insulin-like growth factor1 (IGF-1) at the lesion site, however, had no effect on the expression of pro-inflammatory factors such as TNF-alpha and IL-1 beta. Hence, our data suggested that exogenous intradiencephalon HA retarded locomotor recovery in spinal cord injured zebrafish via modulating the repair microenvironment. CI - Copyright (c) 2017 Elsevier Inc. All rights reserved. FAU - Huang, Shu-Bing AU - Huang SB AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. FAU - Zhao, Hou-De AU - Zhao HD AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. FAU - Wang, Lin-Fang AU - Wang LF AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. FAU - Sun, Meng-Fei AU - Sun MF AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. FAU - Zhu, Ying-Li AU - Zhu YL AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. FAU - Wu, Yi-Bo AU - Wu YB AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China; Affiliated Hospital of Jiangnan University, Human Reproductive and Genetic Center, Wuxi, China. FAU - Xu, Yi-Da AU - Xu YD AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. FAU - Peng, Shi-Xiao AU - Peng SX AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. FAU - Cui, Chun AU - Cui C AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. Electronic address: cuichun@jiangnan.edu.cn. FAU - Shen, Yan-Qin AU - Shen YQ AD - Neuroscience Center, Jiangnan University Medical School, Wuxi, China. Electronic address: shenyanqin@jiangnan.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170527 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 820484N8I3 (Histamine) SB - IM EIN - Biochem Biophys Res Commun. 2017 Oct 21;492(3):528. PMID: 28864060 MH - Animals MH - Histamine/*administration & dosage/*pharmacology MH - Injections, Intraventricular MH - Locomotion/*drug effects MH - Neuroglia/drug effects/metabolism MH - Spinal Cord Injuries/*drug therapy/pathology/*physiopathology MH - Structure-Activity Relationship MH - *Zebrafish/physiology OTO - NOTNLM OT - Diencephalon OT - Histamine OT - Microenvironment OT - Spinal cord injury OT - Zebrafish EDAT- 2017/06/01 06:00 MHDA- 2017/09/26 06:00 CRDT- 2017/06/01 06:00 PHST- 2017/05/16 00:00 [received] PHST- 2017/05/26 00:00 [accepted] PHST- 2017/06/01 06:00 [pubmed] PHST- 2017/09/26 06:00 [medline] PHST- 2017/06/01 06:00 [entrez] AID - S0006-291X(17)31067-7 [pii] AID - 10.1016/j.bbrc.2017.05.158 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 2017 Jul 29;489(3):275-280. doi: 10.1016/j.bbrc.2017.05.158. Epub 2017 May 27.