PMID- 28560013
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 9
IP  - 5
DP  - 2017
TI  - Stromal cell-derived factor-1alpha and transforming growth factor-beta(1) 
      synergistically facilitate migration and chondrogenesis of synovium-derived stem 
      cells through MAPK pathways.
PG  - 2656-2667
AB  - The clinical translation of tissue engineering methods is confined by the limited 
      external cell sources, which is hopefully to be addressed by the cell guidance 
      approach as cytokine-induced homing and differentiation of the patients' 
      autologous cells. Synovium-derived stem cells (SDSCs) are a potent cell source 
      for cartilage restoration due to its intrinsic proximity and tissue-specific 
      chondrogenic capacity. In this study, stromal cell-derived factor-1alpha (SDF-1alpha) in 
      combination with transforming growth factor beta(1) (TGF-beta(1)) were used to induce 
      SDSCs migration and chondrogenesis in vitro. The migration capacity was evaluated 
      by transwell assay and for chondrogenic evaluation, the expression of Sox9, ACAN 
      and COL2A1 were assessed by quantitative RT-PCR while the expression of sulfated 
      GAG and collagen II were evaluated by Alcian Blue stain and immunohistochemistry 
      respectively. Our data showed that SDF-1alpha/CXC chemokine receptor 4 (CXCR4) was 
      involved in SDSCs migration through phosphoinositide 3-kinase (PI3K)/protein 
      kinase B (Akt) pathway. Exogenous TGF-beta(1) enhanced SDF-1alpha-induced SDSCs 
      migration in a concentration and time-dependent manner through CXCR4, evidenced 
      as complete blockage by AMD3100, the CXCR4 antagonist and this effect was 
      mediated by extracellular regulated protein kinases (ERK) activation. Moreover, 
      the addition of SDF-1alpha augmented the TGF-beta(1)-induced SDSCs chondrogenesis, 
      evidenced by the increased pellet sizes and the expressions of COL 2A1, ACAN and 
      Sox9. This effect was related to c-Jun N-terminal kinase (JNK) activation. 
      Collectively, these results suggest that SDF-1alpha and TGF-beta(1) interacts with each 
      other and synergistically enhance the SDSCs migration and chondrogenesis through 
      MAPK pathways.
FAU - Wang, Yiming
AU  - Wang Y
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Chen, Jifei
AU  - Chen J
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Fan, Wenshuai
AU  - Fan W
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Hua, Bingxuan
AU  - Hua B
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Sun, Bolin
AU  - Sun B
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Zhu, Liang
AU  - Zhu L
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Niu, Xinhao
AU  - Niu X
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Yan, Zuoqin
AU  - Yan Z
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
FAU - Guo, Changan
AU  - Guo C
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan UniversityShanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20170515
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5446545
OTO - NOTNLM
OT  - Stromal cell-derived factor-1alpha
OT  - chondrogenesis
OT  - migration
OT  - synovium-derived stem cells
OT  - transforming growth factor-beta1
COIS- None.
EDAT- 2017/06/01 06:00
MHDA- 2017/06/01 06:01
PMCR- 2017/05/15
CRDT- 2017/06/01 06:00
PHST- 2017/03/02 00:00 [received]
PHST- 2017/05/01 00:00 [accepted]
PHST- 2017/06/01 06:00 [entrez]
PHST- 2017/06/01 06:00 [pubmed]
PHST- 2017/06/01 06:01 [medline]
PHST- 2017/05/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2017 May 15;9(5):2656-2667. eCollection 2017.