PMID- 28560197
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2251-6085 (Print)
IS  - 2251-6093 (Electronic)
IS  - 2251-6085 (Linking)
VI  - 46
IP  - 5
DP  - 2017 May
TI  - The Effect of Snail1 Gene Silencing by siRNA in Metastatic Breast Cancer Cell 
      Lines.
PG  - 659-670
AB  - BACKGROUND: Breast cancer is the most common diagnosed cancer among women in the 
      world. Snail1 plays a role in the development of the invasive phenotypes of 
      cancer, neural cell differentiation, cell division and apoptosis in tumor cells. 
      Traces of snail1 in metastasis of breast cancer to bone are observed. The aim of 
      this study was to investigate the effect of specific snail1 siRNAs on the 
      proliferation, migration, induction of apoptosis and cell cycle arrest of 
      MDA-MB-468 cells. METHODS: In 2015, this experimental study was performed on the 
      MDA-MB-468 cell lines in Immunology Research Center, Tabriz University of Medical 
      Sciences. After the design and construction of siRNA, transfection was performed 
      with transfection reagent. The expression levels of mRNA and protein were 
      measured by qRT-PCR and western blot analysis, respectively. The survival of 
      cells was determined by using MTT assay cells, apoptosis using Tunel assay, Cell 
      migration using scratch assay, Cell cycle analysis by Propidium Iodide (PI) DNA 
      staining method using flow cytometry on the MDA-MB-468. RESULTS: Transfection 
      with siRNA significantly suppressed the expression of snail1 gene in 
      dose-dependent manner after 48 h (P<0.0001). Surprisingly, treatment with snail1 
      siRNA arrested cell cycle in S phases (P<0.0001). Moreover, siRNA transfection 
      had effects on breast adenocarcinoma cells and inhibited the migration 
      (P<0.0001), proliferation (P<0.0001) and induced apoptosis (P<0.0016). 
      CONCLUSION: The snail1 can be considered as a potent adjuvant in breast cancer 
      therapy.
FAU - Aletaha, Mansoor
AU  - Aletaha M
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
AD  - Dept. of Pathobiology, Shiraz University, Shiraz, Iran.
FAU - Mansoori, Behzad
AU  - Mansoori B
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Mohammadi, Ali
AU  - Mohammadi A
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Fazeli, Mehdi
AU  - Fazeli M
AD  - Dept. of Pharmacology and Toxicology, School of Veterinary Medicine, Shiraz 
      University, Shiraz, Iran.
FAU - Baradaran, Behzad
AU  - Baradaran B
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Public Health
JT  - Iranian journal of public health
JID - 7505531
PMC - PMC5442279
OTO - NOTNLM
OT  - Breast cancer
OT  - Cell line
OT  - RNA interference
OT  - Snail1
OT  - siRNA
EDAT- 2017/06/01 06:00
MHDA- 2017/06/01 06:01
PMCR- 2017/05/01
CRDT- 2017/06/01 06:00
PHST- 2017/06/01 06:00 [entrez]
PHST- 2017/06/01 06:00 [pubmed]
PHST- 2017/06/01 06:01 [medline]
PHST- 2017/05/01 00:00 [pmc-release]
AID - ijph-46-659 [pii]
PST - ppublish
SO  - Iran J Public Health. 2017 May;46(5):659-670.