PMID- 28560431
OWN - NLM
STAT- MEDLINE
DCOM- 20180515
LR  - 20210208
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 38
IP  - 1
DP  - 2017 Jul
TI  - Overexpression of miR-15b-5p promotes gastric cancer metastasis by regulating 
      PAQR3.
PG  - 352-358
LID - 10.3892/or.2017.5673 [doi]
AB  - Gastric cancer (GC) is the fifth most common cancer in the world, with 952,000 
      new cases diagnosed in 2012. Tumor metastasis is the major cause of cancer 
      recurrence and death. miR-15b-5p has been reported to be dysregulated in numerous 
      types of cancers. However, the role of miR-15b-5p in GC metastasis remains 
      unclear. An miRNA microarray was adopted to analyze the miRNA expression profile. 
      By employing quantitative real-time polymerase chain reaction (qRT-PCR), 
      miR-15b-5p expression levels were detected in GC cell lines, tissues and plasma 
      samples. In addition, the effects of miR-15b-5p on cell proliferation, migration 
      and invasion were studied by applying gain-of-function approaches. Moreover, the 
      target of miR-15b-5p was assessed by dual-luciferase assay, and the mechanism 
      underlying the regulation of GC metastasis by miR-15b-5p was assessed by rescue 
      experiments. The results revealed that miR-15b-5p was upregulated in GC cell 
      lines, tissues and plasma samples. A high plasma level of miR-15b-5p was 
      correlated with distant tumor metastasis. In addition, overexpression of 
      miR‑15b-5p in GC cells promoted cell proliferation, migration, invasion and 
      epithelial-mesenchymal transition (EMT). Moreover, progestin and adipoQ receptor 
      family member 3 (PAQR3) was found to be a direct target of miR-15b-5p and 
      re-expression of PAQR3 in miR-15b-5p-overexpressing GC cells partly attenuated 
      the proliferation, migration and invasion. These findings revealed that 
      miR-15b-5p promotes the metastasis of GC cells through PAQR3 and may represent a 
      potential biomarker of GC.
FAU - Zhao, Chengcheng
AU  - Zhao C
AD  - Central Laboratory, The Affiliated Jiangning Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, P.R. China.
FAU - Li, Yan
AU  - Li Y
AD  - Central Laboratory, The Affiliated Jiangning Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, P.R. China.
FAU - Chen, Gang
AU  - Chen G
AD  - Department of Oncology, The Affiliated Jiangning Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, P.R. China.
FAU - Wang, Fen
AU  - Wang F
AD  - Department of Oncology, The Affiliated Jiangning Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, P.R. China.
FAU - Shen, Zhili
AU  - Shen Z
AD  - Department of Oncology, The Affiliated Jiangning Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, P.R. China.
FAU - Zhou, Rongping
AU  - Zhou R
AD  - Department of Oncology, The Affiliated Jiangning Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170526
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (MIRN15 microRNA, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (PAQR3 protein, human)
SB  - IM
MH  - Apoptosis
MH  - Case-Control Studies
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Epithelial-Mesenchymal Transition
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Lymphatic Metastasis
MH  - Male
MH  - Membrane Proteins/genetics/*metabolism
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - Stomach Neoplasms/genetics/metabolism/*secondary
MH  - Survival Rate
MH  - Tumor Cells, Cultured
EDAT- 2017/06/01 06:00
MHDA- 2018/05/16 06:00
CRDT- 2017/06/01 06:00
PHST- 2016/10/12 00:00 [received]
PHST- 2016/11/23 00:00 [accepted]
PHST- 2017/06/01 06:00 [pubmed]
PHST- 2018/05/16 06:00 [medline]
PHST- 2017/06/01 06:00 [entrez]
AID - 10.3892/or.2017.5673 [doi]
PST - ppublish
SO  - Oncol Rep. 2017 Jul;38(1):352-358. doi: 10.3892/or.2017.5673. Epub 2017 May 26.