PMID- 28560687
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20181113
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Print)
IS  - 0895-8696 (Linking)
VI  - 62
IP  - 2
DP  - 2017 Jun
TI  - HIV-1 gp120 Upregulates Brain-Derived Neurotrophic Factor (BDNF) Expression in 
      BV2 Cells via the Wnt/beta-Catenin Signaling Pathway.
PG  - 199-208
LID - 10.1007/s12031-017-0931-z [doi]
AB  - HIV-1 gp120 plays a critical role in the pathogenesis of HIV-associated pain, but 
      the underlying molecular mechanisms are incompletely understood. This study aims 
      to determine the effect and possible mechanism of HIV-1 gp120 on BDNF expression 
      in BV2 cells (a murine-derived microglial cell line). We observed that gp120 
      (10 ng/ml) activated BV2 cells in cultures and upregulated proBDNF/mBDNF. 
      Furthermore, gp120-treated BV2 also accumulated Wnt3a and beta-catenin, suggesting 
      the activation of the Wnt/beta-catenin pathway. We demonstrated that activation of 
      the pathway by Wnt3a upregulated BDNF expression. In contrast, inhibition of the 
      Wnt/beta-catenin pathway by either DKK1 or IWR-1 attenuated BDNF upregulation 
      induced by gp120 or Wnt3a. These findings collectively suggest that gp120 
      stimulates BDNF expression in BV2 cells via the Wnt/beta-catenin signaling pathway.
FAU - Wang, Yongdi
AU  - Wang Y
AD  - College of Life Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
FAU - Liao, Jinxu
AU  - Liao J
AD  - College of Life Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
FAU - Tang, Shao-Jun
AU  - Tang SJ
AD  - Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 
      Galveston, TX, 77555, USA.
FAU - Shu, Jianhong
AU  - Shu J
AD  - College of Life Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
FAU - Zhang, Wenping
AU  - Zhang W
AUID- ORCID: 0000-0002-3107-8965
AD  - College of Life Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China. 
      Wenpingzhang@zstu.edu.cn.
LA  - eng
GR  - R01 DA036165/DA/NIDA NIH HHS/United States
GR  - R01 NS079166/NS/NINDS NIH HHS/United States
GR  - R01 NS095747/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20170530
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Wnt3A Protein)
RN  - 0 (Wnt3a protein, mouse)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Line
MH  - HIV Envelope Protein gp120/*pharmacology
MH  - Mice
MH  - Microglia/drug effects/metabolism
MH  - Up-Regulation
MH  - *Wnt Signaling Pathway
MH  - Wnt3A Protein/genetics/metabolism
MH  - beta Catenin/metabolism
PMC - PMC6005692
MID - NIHMS974169
OTO - NOTNLM
OT  - BDNF
OT  - HIV-1 gp120
OT  - Microglia
OT  - Wnt/beta-catenin signaling
EDAT- 2017/06/01 06:00
MHDA- 2018/03/27 06:00
PMCR- 2018/06/18
CRDT- 2017/06/01 06:00
PHST- 2017/04/01 00:00 [received]
PHST- 2017/05/08 00:00 [accepted]
PHST- 2017/06/01 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
PHST- 2017/06/01 06:00 [entrez]
PHST- 2018/06/18 00:00 [pmc-release]
AID - 10.1007/s12031-017-0931-z [pii]
AID - 10.1007/s12031-017-0931-z [doi]
PST - ppublish
SO  - J Mol Neurosci. 2017 Jun;62(2):199-208. doi: 10.1007/s12031-017-0931-z. Epub 2017 
      May 30.