PMID- 28561817 OWN - NLM STAT- MEDLINE DCOM- 20170901 LR - 20170901 IS - 2042-650X (Electronic) IS - 2042-6496 (Linking) VI - 8 IP - 6 DP - 2017 Jun 21 TI - Epigallocatechin-3-gallate attenuates cerebral cortex damage and promotes brain regeneration in acrylamide-treated rats. PG - 2275-2282 LID - 10.1039/c6fo01823h [doi] AB - Acrylamide (ACR) is a neurotoxic industrial chemical intermediate, which is also present in food and water. We investigated the neuroprotective effects of epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in green tea, on ACR-treated rat brain. Rats were pre-treated with EGCG for 4 d and then administered ACR and EGCG for 14 d. EGCG increased acetylcholinesterase (AChE) activity and the rate of Nissl-positive cells in ACR-treated rats. Senescence-associated beta-galactosidase (SA-beta-gal) staining indicated that EGCG attenuated ACR-induced senescence. Tumour necrosis factor alpha (TNF-alpha), inducible nitric oxide synthase (iNOS), and cyclooxygenase 2 (COX-2) protein expression indicated that EGCG inhibited ACR-induced inflammation. In addition, immunohistochemical analysis of nestin and brain-derived neurotrophic factor (BDNF) revealed that EGCG promoted brain regeneration in ACR-treated rats. Altogether, our results suggest that EGCG can attenuate ACR-induced brain damage and promote regeneration in the cerebral cortex of rats. Therefore, we hypothesized that EGCG may alleviate ACR-related nerve injury. FAU - He, Yin AU - He Y AD - College of Food, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang City 110866, People's Republic of China. jsjsyau@yeah.net jsjsyau@sina.com 13940433394@163.com tandehongsy@126.com. FAU - Tan, Dehong AU - Tan D FAU - Mi, Yan AU - Mi Y FAU - Zhou, Qian AU - Zhou Q FAU - Ji, Shujuan AU - Ji S LA - eng PT - Journal Article PL - England TA - Food Funct JT - Food & function JID - 101549033 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neuroprotective Agents) RN - 0 (Plant Extracts) RN - 0 (Tea) RN - 20R035KLCI (Acrylamide) RN - 8R1V1STN48 (Catechin) RN - BQM438CTEL (epigallocatechin gallate) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type II) RN - EC 1.14.99.1 (Cyclooxygenase 2) RN - EC 3.2.1.23 (beta-Galactosidase) SB - IM MH - Acrylamide/*toxicity MH - Animals MH - Brain Diseases/*drug therapy/etiology/metabolism/physiopathology MH - Brain-Derived Neurotrophic Factor/metabolism MH - Camellia sinensis/*chemistry MH - Catechin/administration & dosage/*analogs & derivatives MH - Cerebral Cortex/*drug effects/metabolism/physiopathology MH - Cyclooxygenase 2/metabolism MH - Humans MH - Male MH - Neuroprotective Agents/*administration & dosage MH - Nitric Oxide Synthase Type II/metabolism MH - Plant Extracts/*administration & dosage MH - Rats MH - Rats, Sprague-Dawley MH - Regeneration/drug effects MH - Tea/chemistry MH - beta-Galactosidase/metabolism EDAT- 2017/06/01 06:00 MHDA- 2017/09/02 06:00 CRDT- 2017/06/01 06:00 PHST- 2017/06/01 06:00 [pubmed] PHST- 2017/09/02 06:00 [medline] PHST- 2017/06/01 06:00 [entrez] AID - 10.1039/c6fo01823h [doi] PST - ppublish SO - Food Funct. 2017 Jun 21;8(6):2275-2282. doi: 10.1039/c6fo01823h.