PMID- 28562594
OWN - NLM
STAT- MEDLINE
DCOM- 20170818
LR  - 20210224
IS  - 1546-1696 (Electronic)
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 35
IP  - 7
DP  - 2017 Jul
TI  - Guided self-organization and cortical plate formation in human brain organoids.
PG  - 659-666
LID - 10.1038/nbt.3906 [doi]
AB  - Three-dimensional cell culture models have either relied on the self-organizing 
      properties of mammalian cells or used bioengineered constructs to arrange cells 
      in an organ-like configuration. While self-organizing organoids excel at 
      recapitulating early developmental events, bioengineered constructs reproducibly 
      generate desired tissue architectures. Here, we combine these two approaches to 
      reproducibly generate human forebrain tissue while maintaining its 
      self-organizing capacity. We use poly(lactide-co-glycolide) copolymer (PLGA) 
      fiber microfilaments as a floating scaffold to generate elongated embryoid 
      bodies. Microfilament-engineered cerebral organoids (enCORs) display enhanced 
      neuroectoderm formation and improved cortical development. Furthermore, 
      reconstitution of the basement membrane leads to characteristic cortical tissue 
      architecture, including formation of a polarized cortical plate and radial units. 
      Thus, enCORs model the distinctive radial organization of the cerebral cortex and 
      allow for the study of neuronal migration. Our data demonstrate that combining 3D 
      cell culture with bioengineering can increase reproducibility and improve tissue 
      architecture.
FAU - Lancaster, Madeline A
AU  - Lancaster MA
AUID- ORCID: 0000-0003-2324-8853
AD  - IMBA-Institute of Molecular Biotechnology of the Austrian Academy of Science, 
      Vienna, Austria.
AD  - MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.
FAU - Corsini, Nina S
AU  - Corsini NS
AD  - IMBA-Institute of Molecular Biotechnology of the Austrian Academy of Science, 
      Vienna, Austria.
FAU - Wolfinger, Simone
AU  - Wolfinger S
AD  - IMBA-Institute of Molecular Biotechnology of the Austrian Academy of Science, 
      Vienna, Austria.
FAU - Gustafson, E Hilary
AU  - Gustafson EH
AUID- ORCID: 0000-0003-3978-8088
AD  - IMBA-Institute of Molecular Biotechnology of the Austrian Academy of Science, 
      Vienna, Austria.
FAU - Phillips, Alex W
AU  - Phillips AW
AD  - MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.
FAU - Burkard, Thomas R
AU  - Burkard TR
AD  - IMBA-Institute of Molecular Biotechnology of the Austrian Academy of Science, 
      Vienna, Austria.
AD  - IMP-Institute of Molecular Pathology, Vienna, Austria.
FAU - Otani, Tomoki
AU  - Otani T
AD  - Gurdon Institute and Department of Biochemistry, University of Cambridge, Tennis 
      Court Road, Cambridge, UK.
FAU - Livesey, Frederick J
AU  - Livesey FJ
AD  - Gurdon Institute and Department of Biochemistry, University of Cambridge, Tennis 
      Court Road, Cambridge, UK.
FAU - Knoblich, Juergen A
AU  - Knoblich JA
AD  - IMBA-Institute of Molecular Biotechnology of the Austrian Academy of Science, 
      Vienna, Austria.
LA  - eng
GR  - MC_UP_1201/9/MRC_/Medical Research Council/United Kingdom
GR  - MR/L023784/1/MRC_/Medical Research Council/United Kingdom
GR  - MR/L023784/2/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20170531
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
SB  - IM
CIN - Cell Stem Cell. 2017 Aug 3;21(2):157-158. PMID: 28777940
EIN - Nat Biotechnol. 2018 Oct 11;36(10):1016. PMID: 30307926
MH  - Batch Cell Culture Techniques/*methods
MH  - Cells, Cultured
MH  - Guided Tissue Regeneration/methods
MH  - Humans
MH  - Neural Stem Cells/cytology/*physiology
MH  - Neurogenesis/*physiology
MH  - Organ Culture Techniques/methods
MH  - Organoids/cytology/*growth & development
MH  - Prosencephalon/cytology/*growth & development
MH  - Tissue Engineering/*methods
PMC - PMC5824977
MID - EMS76188
COIS- Declaration of competing financial interests M.A.L. and J.A.K. have filed a 
      patent application for use of this technology in future disease modeling and 
      toxicology testing.
EDAT- 2017/06/01 06:00
MHDA- 2017/08/19 06:00
PMCR- 2018/02/23
CRDT- 2017/06/01 06:00
PHST- 2017/04/23 00:00 [received]
PHST- 2017/05/18 00:00 [accepted]
PHST- 2017/06/01 06:00 [pubmed]
PHST- 2017/08/19 06:00 [medline]
PHST- 2017/06/01 06:00 [entrez]
PHST- 2018/02/23 00:00 [pmc-release]
AID - nbt.3906 [pii]
AID - 10.1038/nbt.3906 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2017 Jul;35(7):659-666. doi: 10.1038/nbt.3906. Epub 2017 May 31.