PMID- 28565801
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 13
IP  - 5
DP  - 2017 May
TI  - Inflammatory response associated with choke vessel remodeling in the extended 
      perforator flap model.
PG  - 2012-2018
LID - 10.3892/etm.2017.4205 [doi]
AB  - Ischemic necrosis of the surgical flap is a common complication. The behavior of 
      choke vessels has an important role in skin flap survival. However, the mechanism 
      of choke vessel remodeling has remained elusive. The purpose of the present study 
      was to investigate the possible association between inflammatory responses and 
      choke vessel remodeling in the extended perforator flap model in rats. After flap 
      elevation, the animals were randomly divided into three groups (n=6 in each) for 
      tissue analysis at three, five or seven days after flap surgery. Six additional 
      rats served as a control group (no flap elevation). Tissue samples were collected 
      from the choke zone for histological, western blot and PCR analyses. Monocyte 
      chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-alpha) as 
      inflammatory cytokines were examined in the present study. Histopathological 
      analysis showed that dilation of choke vessels and increased vessel wall 
      thickness was obvious after flap elevation. It also showed edema, inflammation 
      cell aggregation after the operation. Compared with the control group, the 
      protein and mRNA expression levels of MCP-1 and TNF-alpha were significantly 
      increased at days 3, 5 and 7 after flap elevation, while reaching a maximum at 
      day 5. These findings indicated that inflammatory responses may have an important 
      role in choke vessel remodeling. MCP-1 and TNF-alpha may be considered as potential 
      targets for modulating the behavior of choke vessels.
FAU - Qing, Liming
AU  - Qing L
AD  - Department of Orthopaedics, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410008, P.R. China.
FAU - Lei, Pengfei
AU  - Lei P
AD  - Department of Orthopaedics, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410008, P.R. China.
FAU - Tang, Juyu
AU  - Tang J
AD  - Department of Orthopaedics, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410008, P.R. China.
FAU - Wu, Pangfeng
AU  - Wu P
AD  - Department of Orthopaedics, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410008, P.R. China.
FAU - Wang, Long
AU  - Wang L
AD  - Department of Orthopaedics, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410008, P.R. China.
FAU - Xie, Jie
AU  - Xie J
AD  - Department of Orthopaedics, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410008, P.R. China.
FAU - Hu, Yihe
AU  - Hu Y
AD  - Department of Orthopaedics, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410008, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170308
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5443226
OTO - NOTNLM
OT  - biomarkers
OT  - choke vessels
OT  - inflammation
OT  - perforator flap
EDAT- 2017/06/02 06:00
MHDA- 2017/06/02 06:01
PMCR- 2017/03/08
CRDT- 2017/06/02 06:00
PHST- 2016/01/06 00:00 [received]
PHST- 2016/12/23 00:00 [accepted]
PHST- 2017/06/02 06:00 [entrez]
PHST- 2017/06/02 06:00 [pubmed]
PHST- 2017/06/02 06:01 [medline]
PHST- 2017/03/08 00:00 [pmc-release]
AID - ETM-0-0-4205 [pii]
AID - 10.3892/etm.2017.4205 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 May;13(5):2012-2018. doi: 10.3892/etm.2017.4205. Epub 2017 Mar 
      8.