PMID- 28570530
OWN - NLM
STAT- MEDLINE
DCOM- 20180123
LR  - 20190518
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 123
DP  - 2017 May 18
TI  - Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells 
      for Potential Cytoplasm Replacement Therapy.
LID - 10.3791/55741 [doi]
LID - 55741
AB  - We have previously reported on the generation of plasma membrane vesicles (PMVs) 
      through the mechanical extrusion of mammalian cells. The fusion of PMVs with 
      mitochondrial deficient Rho0 cells restored mitotic activity under normal culture 
      conditions. Atherosclerosis, type 2 diabetes, Alzheimer's disease, and cancer are 
      age-related diseases that have been reported to be associated with multiple 
      mechanical and functional defects in the cytosol and organelles of a variety of 
      cell types. Bone marrow mesenchymal stem cells (BMSCs) represent a unique cell 
      population from the bone marrow that possess self-renewal capabilities while 
      maintaining their multipotency. The supplementation of senescence cells with 
      young cytoplasm from autologous BMSCs via the fusion of PMVs provides a promising 
      approach to ameliorate or even reverse age-associated phenotypes. This protocol 
      describes how to prepare PMVs from BMSCs via extrusion through a polycarbonate 
      membrane with 3 microm pores, determine the existence of mitochondria and examine the 
      maintenance of membrane potential within PMVs using a confocal microscope, 
      concentrate PMVs by centrifugation, and carry out the in vivo injection of PMVs 
      into the gastrocnemius muscle of mice.
FAU - Xu, Li-Qun
AU  - Xu LQ
AD  - Multidisciplinary Research Center, Shantou University.
FAU - Lin, Mei-Jia
AU  - Lin MJ
AD  - Multidisciplinary Research Center, Shantou University.
FAU - Li, Yun-Pan
AU  - Li YP
AD  - Multidisciplinary Research Center, Shantou University.
FAU - Li, Shuang
AU  - Li S
AD  - Multidisciplinary Research Center, Shantou University.
FAU - Chen, Shao-Jun
AU  - Chen SJ
AD  - Multidisciplinary Research Center, Shantou University.
FAU - Wei, Chi-Ju
AU  - Wei CJ
AD  - Multidisciplinary Research Center, Shantou University; chijuwei@stu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20170518
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Polycarboxylate Cement)
RN  - 25766-59-0 (polycarbonate)
SB  - IM
MH  - Animals
MH  - Cell Fusion
MH  - Cell Membrane/ultrastructure
MH  - Cell-Derived Microparticles/*transplantation
MH  - Cytoplasm/*transplantation
MH  - Membrane Potential, Mitochondrial
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Polycarboxylate Cement
PMC - PMC5608001
EDAT- 2017/06/02 06:00
MHDA- 2018/01/24 06:00
PMCR- 2019/05/18
CRDT- 2017/06/02 06:00
PHST- 2017/06/02 06:00 [entrez]
PHST- 2017/06/02 06:00 [pubmed]
PHST- 2018/01/24 06:00 [medline]
PHST- 2019/05/18 00:00 [pmc-release]
AID - 55741 [pii]
AID - 10.3791/55741 [doi]
PST - epublish
SO  - J Vis Exp. 2017 May 18;(123):55741. doi: 10.3791/55741.