PMID- 28570746 OWN - NLM STAT- MEDLINE DCOM- 20180319 LR - 20180319 IS - 1521-186X (Electronic) IS - 0197-8462 (Linking) VI - 38 IP - 6 DP - 2017 Sep TI - Influence of extremely low frequency magnetic fields on Ca(2+) signaling and double messenger system in mice hippocampus and reversal function of procyanidins extracted from lotus seedpod. PG - 436-446 LID - 10.1002/bem.22058 [doi] AB - This research investigated the influence of extremely low frequency magnetic fields (ELF-MF; 50 Hz, 8 mT, 4 h per day, for 28 days) on calcium ion signaling and the double messenger system in the hippocampus of mice. Messengers that were studied included: G-protein, Ins(1,4,5)P(3) (IP(3) ), diacylglycerol (DAG), cAMP-dependent protein kinase (PKA), and Ca(2+) -dependent protein kinase C (PKC). The results showed that ELF-MF caused an increase in the levels of Gi protein, IP3, DAG, PKA and PKC beta, calcium and calmodulin-dependent protein phosphatase calcineuring (PP2B), and intracellular Ca(2+) content, and a decrease in calcium/calmodulin-dependent protein kinase II (CaMK II) and PKC alpha. In addition, ELF-MF exposure decreased the level of brain-derived neurotrophic factor (BDNF), which played a key role in hippocampal neuronal cell death. However, oral administration of procyanidins from lotus seedpod (LSPCs) (especially 90 mg kg(-1) ) significantly recovered these changes, and nearly reached normal levels. All these showed that LSPCs may mediate calcium signal and double messenger system through Ca(2+) /CaMK II/CREB/BDNF and DG/PKC/MAPK signaling pathways to reverse the alteration caused by ELF-MF. Bioelectromagnetics. 38:436-446, 2017. (c) 2017 Wiley Periodicals, Inc. CI - (c) 2017 Wiley Periodicals, Inc. FAU - Zhang, Haihui AU - Zhang H AD - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. FAU - Dai, Yuanyuan AU - Dai Y AD - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. FAU - Cheng, Yanxiang AU - Cheng Y AD - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. FAU - He, Yuanqing AU - He Y AD - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. FAU - Manyakara, Zandile AU - Manyakara Z AD - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. FAU - Duan, Yuqing AU - Duan Y AD - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. FAU - Sun, Guibo AU - Sun G AD - Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Beijing, China. FAU - Sun, Xiaobo AU - Sun X AD - Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Beijing, China. LA - eng PT - Journal Article DEP - 20170601 PL - United States TA - Bioelectromagnetics JT - Bioelectromagnetics JID - 8008281 RN - 0 (Biflavonoids) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Diglycerides) RN - 0 (Proanthocyanidins) RN - 4852-22-6 (procyanidin) RN - 85166-31-0 (Inositol 1,4,5-Trisphosphate) RN - 8R1V1STN48 (Catechin) RN - EC 2.7.- (Protein Kinases) RN - EC 3.6.1.- (GTP-Binding Proteins) SB - IM MH - Animals MH - Biflavonoids/isolation & purification/*pharmacology MH - Brain-Derived Neurotrophic Factor/metabolism MH - Calcium Signaling/*drug effects/*radiation effects MH - Catechin/isolation & purification/*pharmacology MH - Diglycerides/metabolism MH - GTP-Binding Proteins/metabolism MH - Hippocampus/*cytology/drug effects/radiation effects MH - Inositol 1,4,5-Trisphosphate/metabolism MH - Magnetic Fields/*adverse effects MH - Magnoliopsida/*chemistry MH - Male MH - Mice MH - Mice, Inbred ICR MH - Proanthocyanidins/isolation & purification/*pharmacology MH - Protein Kinases/metabolism MH - Seeds/*chemistry OTO - NOTNLM OT - Ca2+ signaling OT - double messenger system OT - extremely low frequency magnetic field OT - hippocampus OT - lotus seedpod procyanidins EDAT- 2017/06/02 06:00 MHDA- 2018/03/20 06:00 CRDT- 2017/06/02 06:00 PHST- 2016/03/30 00:00 [received] PHST- 2017/04/22 00:00 [accepted] PHST- 2017/06/02 06:00 [pubmed] PHST- 2018/03/20 06:00 [medline] PHST- 2017/06/02 06:00 [entrez] AID - 10.1002/bem.22058 [doi] PST - ppublish SO - Bioelectromagnetics. 2017 Sep;38(6):436-446. doi: 10.1002/bem.22058. Epub 2017 Jun 1.