PMID- 28573275
OWN - NLM
STAT- MEDLINE
DCOM- 20180329
LR  - 20180329
IS  - 1460-4744 (Electronic)
IS  - 0306-0012 (Linking)
VI  - 46
IP  - 14
DP  - 2017 Jul 17
TI  - Hybridization chain reaction: a versatile molecular tool for biosensing, 
      bioimaging, and biomedicine.
PG  - 4281-4298
LID - 10.1039/c7cs00055c [doi]
AB  - Developing powerful, simple and low-cost DNA amplification techniques is of great 
      significance to bioanalysis and biomedical research. Thus far, many signal 
      amplification strategies have been developed, such as polymerase chain reaction 
      (PCR), rolling circle amplification (RCA), and DNA strand displacement 
      amplification (SDA). In particular, hybridization chain reaction (HCR), a type of 
      toehold-mediated strand displacement (TMSD) reaction, has attracted great 
      interest because of its enzyme-free nature, isothermal conditions, simple 
      protocols, and excellent amplification efficiency. In a typical HCR, an analyte 
      initiates the cross-opening of two DNA hairpins, yielding nicked double helices 
      that are analogous to alternating copolymers. As an efficient amplification 
      platform, HCR has been utilized for the sensitive detection of a wide variety of 
      analytes, including nucleic acids, proteins, small molecules, and cells. In 
      recent years, more complicated sets of monomers have been designed to develop 
      nonlinear HCR, such as branched HCR and even dendritic systems, achieving 
      quadratic and exponential growth mechanisms. In addition, HCR has attracted 
      enormous attention in the fields of bioimaging and biomedicine, including 
      applications in fluorescence in situ hybridization (FISH) imaging, live cell 
      imaging, and targeted drug delivery. In this review, we introduce the 
      fundamentals of HCR and examine the visualization and analysis techniques for HCR 
      products in detail. The most recent HCR developments in biosensing, bioimaging, 
      and biomedicine are subsequently discussed with selected examples. Finally, the 
      review provides insight into the challenges and future perspectives of HCR.
FAU - Bi, Sai
AU  - Bi S
AD  - Collaborative Innovation Center for Marine Biomass Fiber, Materials and Textiles 
      of Shandong Province, College of Chemistry and Chemical Engineering, Shandong 
      Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, 
      Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key 
      Laboratory, Qingdao University, Qingdao 266071, P. R. China.
FAU - Yue, Shuzhen
AU  - Yue S
FAU - Zhang, Shusheng
AU  - Zhang S
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Chem Soc Rev
JT  - Chemical Society reviews
JID - 0335405
SB  - IM
MH  - *Biomedical Research
MH  - *Biosensing Techniques
MH  - Humans
MH  - *Molecular Imaging
MH  - *Nucleic Acid Amplification Techniques
MH  - *Nucleic Acid Hybridization
EDAT- 2017/06/03 06:00
MHDA- 2018/03/30 06:00
CRDT- 2017/06/03 06:00
PHST- 2017/06/03 06:00 [pubmed]
PHST- 2018/03/30 06:00 [medline]
PHST- 2017/06/03 06:00 [entrez]
AID - 10.1039/c7cs00055c [doi]
PST - ppublish
SO  - Chem Soc Rev. 2017 Jul 17;46(14):4281-4298. doi: 10.1039/c7cs00055c.