PMID- 28573506
OWN - NLM
STAT- MEDLINE
DCOM- 20170905
LR  - 20220318
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Print)
IS  - 0163-2116 (Linking)
VI  - 62
IP  - 8
DP  - 2017 Aug
TI  - Estradiol Has Differential Effects on Acute Colonic Inflammation in the Presence 
      and Absence of Estrogen Receptor beta Expression.
PG  - 1977-1984
LID - 10.1007/s10620-017-4631-x [doi]
AB  - BACKGROUND: Inflammatory bowel disease (IBD) increases the risk of developing 
      colon cancer. This risk is higher in men compared to women, implicating a role 
      for female hormones in the protection against this disease. Studies from our 
      laboratory demonstrated that estradiol (E(2)) protects against 
      inflammation-associated colon tumor formation when administered following 
      chemical carcinogen and induction of chronic colitis. AIM: This study seeks to 
      better understand the effect of E(2) on acute colitis in the presence and absence 
      of estrogen receptor beta (ERbeta). METHODS: Inflammation was induced by 
      2,4,6-trinitrobenzenesulfonic acid in wild-type (WT) and ERbeta knockout (ERbetaKO) 
      mice implanted with a control or E(2)-containing pellet and killed 5 days later. 
      Inflammation and injury were scored by a pathologist. Apoptosis and proliferation 
      were assessed by immunohistochemistry. Cytokines were measured by multiplex 
      analysis. RESULTS: E(2) treatment reduced inflammation in the middle colon in WT 
      mice and the distal colon in ERbetaKO mice compared to control mice. WT mice had 
      reduced IL-6, IL-12, IL-17, GM-CSF, IFN-gamma, MCP-1, MIP-1alpha, and TNF-alpha, and ERbetaKO 
      had reduced IL-6 and IFN-gamma expression in response to E(2). Injury scores were 
      lower in E(2)-treated ERbetaKO mice compared to control ERbetaKO mice. ERbetaKO mice had 
      increased proliferation in the basal third of crypts in the distal colon and 
      decreased apoptosis in the proximal colon. CONCLUSIONS: These data suggest that 
      E(2) has differential protective effects against acute colitis in the presence or 
      absence of ERbeta and provide insight into how E(2) may protect against IBD.
FAU - Armstrong, Cameron M
AU  - Armstrong CM
AD  - Department of Nutrition and Food Science, Texas A&M University, 214B Cater 
      Mattil, 2253 TAMU, College Station, TX, 77843, USA.
FAU - Allred, Kimberly F
AU  - Allred KF
AD  - Department of Nutrition and Food Science, Texas A&M University, 214B Cater 
      Mattil, 2253 TAMU, College Station, TX, 77843, USA.
FAU - Weeks, Brad R
AU  - Weeks BR
AD  - Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, 
      USA.
FAU - Chapkin, Robert S
AU  - Chapkin RS
AD  - Department of Nutrition and Food Science, Texas A&M University, 214B Cater 
      Mattil, 2253 TAMU, College Station, TX, 77843, USA.
FAU - Allred, Clinton D
AU  - Allred CD
AD  - Department of Nutrition and Food Science, Texas A&M University, 214B Cater 
      Mattil, 2253 TAMU, College Station, TX, 77843, USA. callred@tamu.edu.
LA  - eng
GR  - P30 ES023512/ES/NIEHS NIH HHS/United States
GR  - R01 CA202697/CA/NCI NIH HHS/United States
GR  - R35 CA197707/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170601
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Cytokines)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 0 (Inflammation Mediators)
RN  - 4TI98Z838E (Estradiol)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cell Proliferation/drug effects
MH  - Colitis/chemically induced/*drug therapy/*metabolism
MH  - Colonic Neoplasms/chemically induced/prevention & control
MH  - Cytokines/analysis/drug effects
MH  - Estradiol/*pharmacology
MH  - Estrogen Receptor beta/analysis/genetics/*metabolism
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Immunohistochemistry
MH  - Inflammation Mediators/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Trinitrobenzenesulfonic Acid
PMC - PMC5751962
MID - NIHMS929742
OTO - NOTNLM
OT  - Colon cancer
OT  - Crohn's disease
OT  - Estradiol
OT  - Estrogen receptor beta
OT  - Inflammation
COIS- Conflict of interest None of the authors have any conflicts of interest to 
      declare.
EDAT- 2017/06/03 06:00
MHDA- 2017/09/07 06:00
PMCR- 2018/01/03
CRDT- 2017/06/03 06:00
PHST- 2017/03/01 00:00 [received]
PHST- 2017/05/24 00:00 [accepted]
PHST- 2017/06/03 06:00 [pubmed]
PHST- 2017/09/07 06:00 [medline]
PHST- 2017/06/03 06:00 [entrez]
PHST- 2018/01/03 00:00 [pmc-release]
AID - 10.1007/s10620-017-4631-x [pii]
AID - 10.1007/s10620-017-4631-x [doi]
PST - ppublish
SO  - Dig Dis Sci. 2017 Aug;62(8):1977-1984. doi: 10.1007/s10620-017-4631-x. Epub 2017 
      Jun 1.