PMID- 28573627
OWN - NLM
STAT- MEDLINE
DCOM- 20180309
LR  - 20180619
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1628
DP  - 2017
TI  - Visualizing Ebolavirus Particles Using Single-Particle Interferometric 
      Reflectance Imaging Sensor (SP-IRIS).
PG  - 259-270
LID - 10.1007/978-1-4939-7116-9_21 [doi]
AB  - This chapter describes an approach for the label-free imaging and quantification 
      of intact Ebola virus (EBOV) and EBOV viruslike particles (VLPs) using a light 
      microscopy technique. In this technique, individual virus particles are captured 
      onto a silicon chip that has been printed with spots of virus-specific capture 
      antibodies. These captured virions are then detected using an optical approach 
      called interference reflectance imaging. This approach allows for the detection 
      of each virus particle that is captured on an antibody spot and can resolve the 
      filamentous structure of EBOV VLPs without the need for electron microscopy. 
      Capture of VLPs and virions can be done from a variety of sample types ranging 
      from tissue culture medium to blood. The technique also allows automated 
      quantitative analysis of the number of virions captured. This can be used to 
      identify the virus concentration in an unknown sample. In addition, this 
      technique offers the opportunity to easily image virions captured from native 
      solutions without the need for additional labeling approaches while offering a 
      means of assessing the range of particle sizes and morphologies in a quantitative 
      manner.
FAU - Carter, Erik P
AU  - Carter EP
AD  - Microbiology and National Emerging Infectious Diseases Laboratories, Boston 
      University School of Medicine, Boston, MA, USA.
FAU - Seymour, Elif C
AU  - Seymour EC
AD  - Biomedical Engineering Department, Boston University, Boston, MA, USA.
FAU - Scherr, Steven M
AU  - Scherr SM
AD  - Mechanical Engineering Department, Boston University, Boston, MA, USA.
FAU - Daaboul, George G
AU  - Daaboul GG
AD  - Electrical and Computer Engineering Department, Boston University, Boston, MA, 
      USA.
FAU - Freedman, David S
AU  - Freedman DS
AD  - NanoView Diagnostics Inc., Boston, MA, USA.
FAU - Selim Unlu, M
AU  - Selim Unlu M
AD  - Biomedical Engineering Department, Boston University, Boston, MA, USA.
AD  - Mechanical Engineering Department, Boston University, Boston, MA, USA.
AD  - Electrical and Computer Engineering Department, Boston University, Boston, MA, 
      USA.
AD  - Photonics Center, Boston University, Boston, MA, USA.
FAU - Connor, John H
AU  - Connor JH
AD  - Microbiology and National Emerging Infectious Diseases Laboratories, Boston 
      University School of Medicine, Boston, MA, USA. jhconnor@bu.edu.
AD  - Biomedical Engineering Department, Boston University, Boston, MA, USA. 
      jhconnor@bu.edu.
LA  - eng
GR  - R01 AI096159/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Ebolavirus/*isolation & purification/pathogenicity
MH  - Hemorrhagic Fever, Ebola/*diagnostic imaging/virology
MH  - Humans
MH  - Interferometry/*methods
MH  - Virion/isolation & purification/pathogenicity
OTO - NOTNLM
OT  - Biosensing
OT  - Ebola
OT  - High throughput
OT  - Interferometric imaging
OT  - Label-free
OT  - Viral hemorrhagic fevers
OT  - Virus detection
EDAT- 2017/06/03 06:00
MHDA- 2018/03/10 06:00
CRDT- 2017/06/03 06:00
PHST- 2017/06/03 06:00 [entrez]
PHST- 2017/06/03 06:00 [pubmed]
PHST- 2018/03/10 06:00 [medline]
AID - 10.1007/978-1-4939-7116-9_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1628:259-270. doi: 10.1007/978-1-4939-7116-9_21.