PMID- 28575983
OWN - NLM
STAT- MEDLINE
DCOM- 20180309
LR  - 20181202
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 78
DP  - 2017 Sep 1
TI  - Evaluation of nanohydroxyapaptite (nano-HA) coated epigallocatechin-3-gallate 
      (EGCG) cross-linked collagen membranes.
PG  - 258-264
LID - S0928-4931(17)31159-1 [pii]
LID - 10.1016/j.msec.2017.04.069 [doi]
AB  - Collagen is the main component of extracellular matrix (ECM) with desirable 
      biological activities and low antigenicity. Collagen materials have been widely 
      utilized in guided bone regeneration (GBR) surgery due to its abilities to 
      maintain space for hard tissue growth. However, pure collagen lacks optimal 
      mechanical properties. In our previous study, epigallocatechin-3-gallate (EGCG) 
      cross-linked collagen membranes, with better biological activities and enhanced 
      mechanical properties, may promote osteoblast proliferation, but their effect on 
      osteoblast differentiation is not very significant. Nanohydroxyapatite (nano-HA) 
      is the main component of mineral bone, which possesses exceptional bioactivity 
      properties including good biocompatibility, high osteoconductivity and 
      osteoinductivity, non-immunogenicity and non-inflammatory behavior. Herein, by 
      analyzing the physical and chemical properties as well as the effects on 
      promoting bone regeneration, we have attempted to present a novel EGCG-modified 
      collagen membrane with nano-HA coating, and have found evidence that the novel 
      collagen membrane may promote bone regeneration with a better surface morphology, 
      without destroying collagen backbone. To evaluate the surface morphologies, 
      chemical and mechanical properties of pure collagen membranes, 
      epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes, nano-HA coated 
      collagen membranes, nano-HA coated EGCG-collagen membranes, (ii) to evaluate the 
      bone regeneration promoted by theses membranes. In the present study, collagen 
      membranes were divided into 4 groups: (1) untreated collagen membranes (2) EGCG 
      cross-linked collagen membranes (3) nano-HA modified collagen membranes (4) 
      nano-HA modified EGCG-collagen membranes. Scanning electron microscope (SEM) and 
      Fourier transform infrared spectroscopy (FTIR) were used to evaluate surface 
      morphologies and chemical properties, respectively. Mechanical properties were 
      determined by differential scanning calorimeter (DSC) and elastic modulus (EM) 
      measurements. Then in 12 rats, 4 types of membranes were randomly applied to 
      cover the rat calvarial defects. The animals were sacrificed at 8weeks. 
      Histologic analyses were performed using Hematoxylin-eosin (H&E) staining and 
      Masson's Trichrome stains. For statistical analysis, analysis of variance (ANOVA) 
      followed by Tukey's multiple comparison tests was applied. HA nanoparticles were 
      fairly well distributed nanoparticles among the collagen fibers on the 
      nano-HA-modified EGCG-collagen membranes, with smoother surface. Moreover, 
      collagen membranes with modifications all maintained their collagen backbone and 
      the mechanical properties were enhanced by EGCG and nano-HA treatments. In 
      addition, EGCG cross-linked collagen membranes with nano-HA coatings promoted 
      bone regeneration. Nano-HA modified EGCG-collagen membranes can be utilized as a 
      barrier membrane to enhance the bone regeneration in GBR surgeries.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Chu, Chenyu
AU  - Chu C
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu 610041, China.
FAU - Deng, Jia
AU  - Deng J
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu 610041, China.
FAU - Man, Yi
AU  - Man Y
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu 610041, China; Department of Oral Implantology, West 
      China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. 
      Electronic address: manyi780203@126.com.
FAU - Qu, Yili
AU  - Qu Y
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu 610041, China. Electronic address: qqyili@126.com.
LA  - eng
PT  - Journal Article
DEP - 20170418
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Membranes, Artificial)
RN  - 8R1V1STN48 (Catechin)
RN  - 9007-34-5 (Collagen)
RN  - 91D9GV0Z28 (Durapatite)
RN  - BQM438CTEL (epigallocatechin gallate)
SB  - IM
MH  - Animals
MH  - Bone Regeneration
MH  - Catechin/analogs & derivatives
MH  - Collagen/*chemistry
MH  - Durapatite
MH  - Membranes, Artificial
MH  - Osteogenesis
MH  - Rats
OTO - NOTNLM
OT  - Biomaterials
OT  - Collagen membrane
OT  - Guide bone regeneration
EDAT- 2017/06/04 06:00
MHDA- 2018/03/10 06:00
CRDT- 2017/06/04 06:00
PHST- 2017/03/30 00:00 [received]
PHST- 2017/04/11 00:00 [revised]
PHST- 2017/04/13 00:00 [accepted]
PHST- 2017/06/04 06:00 [entrez]
PHST- 2017/06/04 06:00 [pubmed]
PHST- 2018/03/10 06:00 [medline]
AID - S0928-4931(17)31159-1 [pii]
AID - 10.1016/j.msec.2017.04.069 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:258-264. doi: 
      10.1016/j.msec.2017.04.069. Epub 2017 Apr 18.