PMID- 28577058
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20190729
IS  - 1435-1463 (Electronic)
IS  - 0300-9564 (Linking)
VI  - 124
IP  - 9
DP  - 2017 Sep
TI  - Type B and A monoamine oxidase and their inhibitors regulate the gene expression 
      of Bcl-2 and neurotrophic factors in human glioblastoma U118MG cells: different 
      signal pathways for neuroprotection by selegiline and rasagiline.
PG  - 1055-1066
LID - 10.1007/s00702-017-1740-9 [doi]
AB  - Type B monoamine oxidase (MAO-B) in glial cells has been considered to be 
      associated with neuronal death in Parkinson's disease. MAO-B inhibitors, 
      rasagiline and selegiline [(-)deprenyl], protect neurons in animal and cellular 
      models of neurodegeneration. However, the role of MAO-B itself in the regulation 
      of cell death processing remains elusive, whereas type A MAO (MAO-A) mediates the 
      induction of anti-apoptotic Bcl-2 genes by rasagiline and selegiline. In this 
      paper, the involvement of MAOs in the induction of neuroprotective genes by MAO 
      inhibitors was investigated in human glioblastoma U118MG cells expressing mainly 
      MAO-B. Selegiline significantly increased Mao-B, which was suppressed by Mao-A 
      knockdown with short interfering (si)RNA, whereas rasagiline less markedly 
      increased Mao-B, which was not affected by Mao-A knockdown. Mao-A mRNA was also 
      markedly increased by rasagiline and selegiline, and Mao-B knockdown 
      significantly enhanced the induction by selegiline, but not by rasagiline. Mao-B 
      knockdown also significantly increased mRNA levels of Bcl-2, brain-derived 
      neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor 
      (GDNF). Selegiline synergistically enhanced the expression of these genes in 
      Mao-B knockdown cells, but Mao-A knockdown suppressed the increase. Rasagiline 
      increased BDNF and GDNF, which Mao-B and Mao-A knockdown inhibited. These results 
      show that MAO-B might function as a repressor and MAO-A as a mediator in the 
      constitutional expression of pro-survival genes, and that MAO-B and MAO-A might 
      regulate different signal pathways for rasagiline and selegiline to induce 
      neuroprotective genes. The novel role of glial MAOs in the regulation of gene 
      expression is discussed.
FAU - Inaba-Hasegawa, Keiko
AU  - Inaba-Hasegawa K
AD  - Department of Health and Nutrition, Faculty of Psychological and Physical 
      Science, Aichi Gakuin University, 12 Araike, Iwasaki-cho, Nisshin, Aichi, 
      470-0195, Japan.
FAU - Shamoto-Nagai, Masayo
AU  - Shamoto-Nagai M
AD  - Department of Health and Nutrition, Faculty of Psychological and Physical 
      Science, Aichi Gakuin University, 12 Araike, Iwasaki-cho, Nisshin, Aichi, 
      470-0195, Japan.
FAU - Maruyama, Wakako
AU  - Maruyama W
AD  - Department of Health and Nutrition, Faculty of Psychological and Physical 
      Science, Aichi Gakuin University, 12 Araike, Iwasaki-cho, Nisshin, Aichi, 
      470-0195, Japan.
FAU - Naoi, Makoto
AU  - Naoi M
AD  - Department of Health and Nutrition, Faculty of Psychological and Physical 
      Science, Aichi Gakuin University, 12 Araike, Iwasaki-cho, Nisshin, Aichi, 
      470-0195, Japan. mnaoi@dpc.agu.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20170602
PL  - Austria
TA  - J Neural Transm (Vienna)
JT  - Journal of neural transmission (Vienna, Austria : 1996)
JID - 9702341
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Indans)
RN  - 0 (Monoamine Oxidase Inhibitors)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 003N66TS6T (rasagiline)
RN  - 2K1V7GP655 (Selegiline)
RN  - EC 1.4.3.4 (Monoamine Oxidase)
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/drug effects/physiology
MH  - Astrocytes/drug effects/enzymology
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Gene Knockdown Techniques
MH  - Glioblastoma/*drug therapy/*enzymology
MH  - Humans
MH  - Indans/*pharmacology
MH  - Monoamine Oxidase/genetics/*metabolism
MH  - Monoamine Oxidase Inhibitors/pharmacology
MH  - Nerve Growth Factors/metabolism
MH  - Neuroprotection/drug effects/physiology
MH  - Neuroprotective Agents/*pharmacology
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - RNA, Messenger/metabolism
MH  - Selegiline/*pharmacology
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Bcl-2
OT  - MAO-B inhibitor rasagiline-selegiline
OT  - Neuroprotective gene induction
OT  - Neurotrophic factors BDNF-GDNF
OT  - Type B and A monoamine oxidase
EDAT- 2017/06/04 06:00
MHDA- 2019/07/30 06:00
CRDT- 2017/06/04 06:00
PHST- 2017/03/29 00:00 [received]
PHST- 2017/05/30 00:00 [accepted]
PHST- 2017/06/04 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2017/06/04 06:00 [entrez]
AID - 10.1007/s00702-017-1740-9 [pii]
AID - 10.1007/s00702-017-1740-9 [doi]
PST - ppublish
SO  - J Neural Transm (Vienna). 2017 Sep;124(9):1055-1066. doi: 
      10.1007/s00702-017-1740-9. Epub 2017 Jun 2.